Immunological response to infection with human reovirus-like agent: measurement of anti-human reovirus-like agent immunoglobulin G and M levels by the method of enzyme-linked immunosorbent assay

Robert H. Yolken; Richard G. Wyatt; Hyun W. Kim; Albert Z. Kapikian; Robert M. Chanock

doi:10.1128/iai.19.2.540-546.1978

Immunological response to infection with human reovirus-like agent: measurement of anti-human reovirus-like agent immunoglobulin G and M levels by the method of enzyme-linked immunosorbent assay

Robert H. Yolken, Richard G. Wyatt, Hyun W. Kim, Albert Z. Kapikian, Robert M. Chanock

Research output: Contribution to journal › Article › peer-review

41 Scopus citations

Abstract

The report describes the development of the enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against the human reovirus-like agent of infantile gastroenteritis (HRVLA). This ELISA system proved to be four times as sensitive as the standard anti-HRVLA fluorescent-antibody assay and ten times as sensitive as the standard anti-HRVLA complement fixation assay. In addition, the ELISA was capable of determining immunoglobulin G (IgG) and IgM subclasses of anti-HRVLA antibody using a single dilution of serum. With this assay, it was discovered that 11 of 21 infected children had anti-HRVLA IgM in their acute sera before the appearance of anti-HRVLA IgG. ELISA is a useful tool in the evaluation of immunological response to HRVLA infection.

Original language	English (US)
Pages (from-to)	540-546
Number of pages	7
Journal	Unknown Journal
Volume	19
Issue number	2
DOIs	https://doi.org/10.1128/iai.19.2.540-546.1978
State	Published - 1978
Externally published	Yes

ASJC Scopus subject areas

Parasitology
Microbiology
Immunology
Infectious Diseases

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10.1128/iai.19.2.540-546.1978

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Immunological response to infection with human reovirus-like agent: measurement of anti-human reovirus-like agent immunoglobulin G and M levels by the method of enzyme-linked immunosorbent assay. / Yolken, Robert H.; Wyatt, Richard G.; Kim, Hyun W. et al.
In: Unknown Journal, Vol. 19, No. 2, 1978, p. 540-546.

Research output: Contribution to journal › Article › peer-review

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abstract = "The report describes the development of the enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against the human reovirus-like agent of infantile gastroenteritis (HRVLA). This ELISA system proved to be four times as sensitive as the standard anti-HRVLA fluorescent-antibody assay and ten times as sensitive as the standard anti-HRVLA complement fixation assay. In addition, the ELISA was capable of determining immunoglobulin G (IgG) and IgM subclasses of anti-HRVLA antibody using a single dilution of serum. With this assay, it was discovered that 11 of 21 infected children had anti-HRVLA IgM in their acute sera before the appearance of anti-HRVLA IgG. ELISA is a useful tool in the evaluation of immunological response to HRVLA infection.",

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AU - Kapikian, Albert Z.

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Immunological response to infection with human reovirus-like agent: measurement of anti-human reovirus-like agent immunoglobulin G and M levels by the method of enzyme-linked immunosorbent assay

Abstract

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