Immunological heterogeneity of human monocytes subsets prepared by counterflow centrifugation elutriation

A. H. Esa, S. J. Noga, A. D. Donnenberg, A. D. Hess

Research output: Contribution to journalArticle

Abstract

Human peripheral blood mononuclear cells were separated into three fractions by means of counterflow centrifugation elutriation (CCE). The first fraction, eluted at a flow rate of 24 ml/min, was composed of lymphocytes with less than 2% contaminating esterase-positive cells. The cells in this fraction were incapable of responding to either soluble antigen (tetanus toxoid) or particulate antigen (cytomegalovirus-infected fibroblasts) unless recombined with accessory cells. The second fraction, eluted at a flow rate of 28 ml/min, was composed predominantly (72%) of small Ia, leu M3, and esterase-positive monocytes, which stained weakly with leu 10 antibody. Cells in this fraction efficiently presented soluble and particulate antigens to monocyte-depleted lymphocytes. Of the remaining cells, 87% were large esterase-positive monocytes that labelled strongly with Ia, leu M3, and leu 10. These cells were less efficient in antigen presentation than the small monocytes. However, lymphocytes activated with antigen-pulsed large monocytes exhibited more suppressor cell activity than those activated with antigen-pulsed small monocytes.

Original languageEnglish (US)
Pages (from-to)95-99
Number of pages5
JournalImmunology
Volume59
Issue number1
StatePublished - Jan 1 1986

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Esa, A. H., Noga, S. J., Donnenberg, A. D., & Hess, A. D. (1986). Immunological heterogeneity of human monocytes subsets prepared by counterflow centrifugation elutriation. Immunology, 59(1), 95-99.