Immunologic properties of mast cells from rats infected with Nippostrongylus brasiliensis

T. Ishizaka, W. Konig, M. Kurata, L. Mauser

Research output: Contribution to journalArticle

Abstract

The concentration of IgE in the serum of Sprague Dawley rats increased after infection with Nippostrongylus brasiliensis (NB). The IgE concentration in normal rats was less than 1 μg/ml. After reinfection with NB, the concentration increased to 100 to 300 μg/ml. Mast cells were purified from peritoneal cells of both normal and NB infected animals. Purified mast cells from the infected animals released histamine upon exposure to NB antigen. The antibody specific for IgE released histamine from purified mast cells of both normal and infected animals. Dose response curves of histamine release suggested that mast cells from NB infected animals bear more IgE molecules than normal mast cells. Binding of 125I labeled rat E myeloma protein with normal mast cells was demonstrated by autoradiography. Under the same experimental conditions, mast cells of infected animals were not labeled with 125I IgE. Mast cells from both normal and infected animals failed to combine 125I labeled IgG. The number of IgE molecules bound per mast cell was determined by incubating 125I labeled IgE with purified mast cells. When mast cells were incubated in 0.6 to 2 μg/ml of IgE, the number of IgE molecules combined with the mast cells from infected animals was about 10% of that bound with normal mast cells. The results indicated that a large proportion of IgE receptors on mast cells of infected animals was occupied by their own IgE. No significant difference was observed between normal mast cells and those of infected animals with respect to histamine content and intracellular levels of cyclic nucleotides.

Original languageEnglish (US)
Pages (from-to)1078-1083
Number of pages6
JournalJournal of Immunology
Volume115
Issue number4
StatePublished - 1975

Fingerprint

Nippostrongylus
Mast Cells
Immunoglobulin E
Histamine
Myeloma Proteins
IgE Receptors
Histamine Release
Cyclic Nucleotides

ASJC Scopus subject areas

  • Immunology

Cite this

Ishizaka, T., Konig, W., Kurata, M., & Mauser, L. (1975). Immunologic properties of mast cells from rats infected with Nippostrongylus brasiliensis. Journal of Immunology, 115(4), 1078-1083.

Immunologic properties of mast cells from rats infected with Nippostrongylus brasiliensis. / Ishizaka, T.; Konig, W.; Kurata, M.; Mauser, L.

In: Journal of Immunology, Vol. 115, No. 4, 1975, p. 1078-1083.

Research output: Contribution to journalArticle

Ishizaka, T, Konig, W, Kurata, M & Mauser, L 1975, 'Immunologic properties of mast cells from rats infected with Nippostrongylus brasiliensis', Journal of Immunology, vol. 115, no. 4, pp. 1078-1083.
Ishizaka, T. ; Konig, W. ; Kurata, M. ; Mauser, L. / Immunologic properties of mast cells from rats infected with Nippostrongylus brasiliensis. In: Journal of Immunology. 1975 ; Vol. 115, No. 4. pp. 1078-1083.
@article{c5e1523163ab4fab899754f6315d39b6,
title = "Immunologic properties of mast cells from rats infected with Nippostrongylus brasiliensis",
abstract = "The concentration of IgE in the serum of Sprague Dawley rats increased after infection with Nippostrongylus brasiliensis (NB). The IgE concentration in normal rats was less than 1 μg/ml. After reinfection with NB, the concentration increased to 100 to 300 μg/ml. Mast cells were purified from peritoneal cells of both normal and NB infected animals. Purified mast cells from the infected animals released histamine upon exposure to NB antigen. The antibody specific for IgE released histamine from purified mast cells of both normal and infected animals. Dose response curves of histamine release suggested that mast cells from NB infected animals bear more IgE molecules than normal mast cells. Binding of 125I labeled rat E myeloma protein with normal mast cells was demonstrated by autoradiography. Under the same experimental conditions, mast cells of infected animals were not labeled with 125I IgE. Mast cells from both normal and infected animals failed to combine 125I labeled IgG. The number of IgE molecules bound per mast cell was determined by incubating 125I labeled IgE with purified mast cells. When mast cells were incubated in 0.6 to 2 μg/ml of IgE, the number of IgE molecules combined with the mast cells from infected animals was about 10{\%} of that bound with normal mast cells. The results indicated that a large proportion of IgE receptors on mast cells of infected animals was occupied by their own IgE. No significant difference was observed between normal mast cells and those of infected animals with respect to histamine content and intracellular levels of cyclic nucleotides.",
author = "T. Ishizaka and W. Konig and M. Kurata and L. Mauser",
year = "1975",
language = "English (US)",
volume = "115",
pages = "1078--1083",
journal = "Journal of Immunology",
issn = "0022-1767",
publisher = "American Association of Immunologists",
number = "4",

}

TY - JOUR

T1 - Immunologic properties of mast cells from rats infected with Nippostrongylus brasiliensis

AU - Ishizaka, T.

AU - Konig, W.

AU - Kurata, M.

AU - Mauser, L.

PY - 1975

Y1 - 1975

N2 - The concentration of IgE in the serum of Sprague Dawley rats increased after infection with Nippostrongylus brasiliensis (NB). The IgE concentration in normal rats was less than 1 μg/ml. After reinfection with NB, the concentration increased to 100 to 300 μg/ml. Mast cells were purified from peritoneal cells of both normal and NB infected animals. Purified mast cells from the infected animals released histamine upon exposure to NB antigen. The antibody specific for IgE released histamine from purified mast cells of both normal and infected animals. Dose response curves of histamine release suggested that mast cells from NB infected animals bear more IgE molecules than normal mast cells. Binding of 125I labeled rat E myeloma protein with normal mast cells was demonstrated by autoradiography. Under the same experimental conditions, mast cells of infected animals were not labeled with 125I IgE. Mast cells from both normal and infected animals failed to combine 125I labeled IgG. The number of IgE molecules bound per mast cell was determined by incubating 125I labeled IgE with purified mast cells. When mast cells were incubated in 0.6 to 2 μg/ml of IgE, the number of IgE molecules combined with the mast cells from infected animals was about 10% of that bound with normal mast cells. The results indicated that a large proportion of IgE receptors on mast cells of infected animals was occupied by their own IgE. No significant difference was observed between normal mast cells and those of infected animals with respect to histamine content and intracellular levels of cyclic nucleotides.

AB - The concentration of IgE in the serum of Sprague Dawley rats increased after infection with Nippostrongylus brasiliensis (NB). The IgE concentration in normal rats was less than 1 μg/ml. After reinfection with NB, the concentration increased to 100 to 300 μg/ml. Mast cells were purified from peritoneal cells of both normal and NB infected animals. Purified mast cells from the infected animals released histamine upon exposure to NB antigen. The antibody specific for IgE released histamine from purified mast cells of both normal and infected animals. Dose response curves of histamine release suggested that mast cells from NB infected animals bear more IgE molecules than normal mast cells. Binding of 125I labeled rat E myeloma protein with normal mast cells was demonstrated by autoradiography. Under the same experimental conditions, mast cells of infected animals were not labeled with 125I IgE. Mast cells from both normal and infected animals failed to combine 125I labeled IgG. The number of IgE molecules bound per mast cell was determined by incubating 125I labeled IgE with purified mast cells. When mast cells were incubated in 0.6 to 2 μg/ml of IgE, the number of IgE molecules combined with the mast cells from infected animals was about 10% of that bound with normal mast cells. The results indicated that a large proportion of IgE receptors on mast cells of infected animals was occupied by their own IgE. No significant difference was observed between normal mast cells and those of infected animals with respect to histamine content and intracellular levels of cyclic nucleotides.

UR - http://www.scopus.com/inward/record.url?scp=0016751870&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0016751870&partnerID=8YFLogxK

M3 - Article

C2 - 51873

AN - SCOPUS:0016751870

VL - 115

SP - 1078

EP - 1083

JO - Journal of Immunology

JF - Journal of Immunology

SN - 0022-1767

IS - 4

ER -