Thirty male Lewis rats underwent vasectomy with ligation of the vas deferens, vasectomy without ligation of the vas deferens, and sham vasectomy in groups of 10. Assays for cell mediated immunity were performed with each experimental group 90 days after operative procedure. Three specific tests were utilized to demonstrate cell mediated immunity specific to syngeneic spermatozoa. The 3 tests were: footpad swelling response to the injection of syngeneic spermatozoa; release of radioactivity from 51chromium labeled spermatozoa; and release of macrophage migration inhibition factor by lymphocytes incubated with spermatozoa. Cell mediated immunity was present only in rats without ligation of the vas deferens.
|Original language||English (US)|
|Number of pages||7|
|Journal||Fertility and Sterility|
|Publication status||Published - 1974|
ASJC Scopus subject areas
- Obstetrics and Gynecology