Immunolocalization of glyceraldehyde-3-phosphate dehydrogenase in Arabidopsis thaliana

Q. Wang, L. Kuo, R. Sjölund, M. C. Shih

Research output: Contribution to journalArticle

Abstract

NAD-dependent glyceraldehyde-3-phosphate dehydrogenase (NAD-dependent GAPDH) was purified to homogeneity and injected into a rabbit to induce a polyclonal antibody. The antibody was judged to be of high specificity and high affinity. This antibody was used to probe sections of Arabidopsis leaf, stem or roots which were fixed using either paraformaldehyde or a high-pressure freezing method. Our results show that the NAD-dependent GAPDH localizes in the nucleus as well as in the cytosol. In phloem tissue, the NAD-dependent GAPDH was found in companion cells but not in the sieve element.

Original languageEnglish (US)
Pages (from-to)155-162
Number of pages8
JournalProtoplasma
Volume198
Issue number3-4
DOIs
StatePublished - Jan 1 1997
Externally publishedYes

Keywords

  • Antibody
  • Arabidopsis thaliana
  • Companion cell
  • Glyceraldehyde-3-phosphate dehydrogenase
  • Phloem
  • Sieve element

ASJC Scopus subject areas

  • Plant Science
  • Cell Biology

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