Immunoliposomal delivery of 213Bi for α-emitter targeting of metastatic breast cancer

Mohanambe Lingappa, Hong Song, Sarah Thompson, Frank Bruchertseifer, Alfred Morgenstern, George Sgouros

Research output: Contribution to journalArticlepeer-review

Abstract

Current treatment for late-stage metastatic breast cancer is largely palliative. α-Particles are highly potent, short-range radiation emissions capable of sterilizing individual cells with one to three traversals of the cell nucleus. The α-emitter, 213Bi (T1/2 = 45.6 min), was conjugated to a 100-nm diameter liposomal-CHX-A″-DTPA construct, upon which the rat HER2/neu reactive antibody, 7.16.4, was grafted. A conjugation time of 10 minutes was achieved giving a specific activity corresponding to 0.1 213Bi atom per liposome; stability in vitro and in vivo was confirmed. Efficacy in a rat/neu transgenic mouse model of metastatic mammary carcinoma was investigated. Three days after left cardiac ventricular injection of 105 rat HER-2/neu-expressing syngeneic tumor cells, macrophage-depleted Neu-N mice were treated by i.v. injection with (a) 19.2 MBq (520 μCi) of liposome-CHX-A″-DTPA-213Bi, (b) 19.2 MBq of liposome-CHX-A″-DTPA-213Bi-7.16.4, (c) 4.44 MBq (120 μCi) of 213Bi-7.16.4, and (d) cold (nonradioactive) liposome-CHX-A″-DTPA-7.16.4 as control. Treatment with (a) increased median survival time to 34 days compared with 29 days for the untreated controls (P = 0.013) and 27 days for treated cold controls. Treatment with the radiolabeled antibody-conjugated liposome (b) increased median survival time to 38 days (P = 0.0002 relative to untreated controls). The radiolabeled antibody-treated group (c) gave a median survival of 39 days, which was similar to that for the radiolabeled antibody-conjugated liposome-treated group (P = 0.5). We have shown that the 213Bi radiolabeled immunoliposomes are effective in treating early-stage micrometastases, giving median survival times similar to those obtained with antibody-mediated delivery of 213Bi in this animal model.

Original languageEnglish (US)
Pages (from-to)6815-6823
Number of pages9
JournalCancer Research
Volume70
Issue number17
DOIs
StatePublished - Sep 1 2010

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Fingerprint Dive into the research topics of 'Immunoliposomal delivery of <sup>213</sup>Bi for α-emitter targeting of metastatic breast cancer'. Together they form a unique fingerprint.

Cite this