Immunohistological approaches to human prostatic epithelial cells.

B. K. Choe, J. E. Pontes, H. S. Lillehoj, N. R. Rose

Research output: Contribution to journalArticle

Abstract

Specific anti-acid phosphatase antisera were prepared by the use of purified prostatic acid phosphatase (PAP) from prostatic tissue and lysosomal acid phosphatase (L-AcP) from diploid fibroblast (WI-38) as antigen. Since the antigenic difference between the PAP and L-AcP was established, distribution of these enzymes among various tissues and tissue culture cells was investigated by the indirect immunofluorescence method and the unlabeled antibody method [14]. The L-AcP has been detected as granules localized in a perinuclear portion of all of the epithelial cells, fibroblasts, and peripheral blood cells except erythrocytes. Presence of the PAP was limited to the prostatic epithelial cells. It is also noted that the biosynthesis of the PAP is strikingly diminished in the long-term culture of prostatic epithelial cells. However, because of the antigenic difference between the PAP and L-AcP, and because of the high concentration of PAP in the cytoplasm of prostatic epithelial cells, it is feasible to apply the anti-PAP immunofluorescence method to the identification of metastatic prostatic carcinoma cells in the biopsy materials.

Original languageEnglish (US)
Pages (from-to)383-398
Number of pages16
JournalProstate
Volume1
Issue number4
StatePublished - 1980
Externally publishedYes

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Epithelial Cells
Acid Phosphatase
Fibroblasts
Indirect Fluorescent Antibody Technique
prostatic acid phosphatase
Diploidy
Fluorescent Antibody Technique
Immune Sera
Blood Cells
Cytoplasm
Cell Culture Techniques
Erythrocytes
Carcinoma
Biopsy
Antigens
Antibodies
Enzymes

ASJC Scopus subject areas

  • Urology

Cite this

Choe, B. K., Pontes, J. E., Lillehoj, H. S., & Rose, N. R. (1980). Immunohistological approaches to human prostatic epithelial cells. Prostate, 1(4), 383-398.

Immunohistological approaches to human prostatic epithelial cells. / Choe, B. K.; Pontes, J. E.; Lillehoj, H. S.; Rose, N. R.

In: Prostate, Vol. 1, No. 4, 1980, p. 383-398.

Research output: Contribution to journalArticle

Choe, BK, Pontes, JE, Lillehoj, HS & Rose, NR 1980, 'Immunohistological approaches to human prostatic epithelial cells.', Prostate, vol. 1, no. 4, pp. 383-398.
Choe BK, Pontes JE, Lillehoj HS, Rose NR. Immunohistological approaches to human prostatic epithelial cells. Prostate. 1980;1(4):383-398.
Choe, B. K. ; Pontes, J. E. ; Lillehoj, H. S. ; Rose, N. R. / Immunohistological approaches to human prostatic epithelial cells. In: Prostate. 1980 ; Vol. 1, No. 4. pp. 383-398.
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