Immunohistochemistry for ERG expression as a surrogate for TMPRSS2-ERG fusion detection in prostatic adenocarcinomas

Alcides Chaux, Roula Albadine, Antoun Toubaji, Jessica Hicks, Alan Keith Meeker, Elizabeth A Platz, Angelo Michael Demarzo, George J. Netto

Research output: Contribution to journalArticle

Abstract

Background: TMPRSS2-ERG fusions have been identified in about one-half of all prostatic adenocarcinomas (PCas). Fluorescence in situ hybridization (FISH) and reverse transcription polymerase chain reaction have been the most commonly used techniques in this setting. The aim of this study was to evaluate the utility of ERG immunoexpression as a surrogate for TMPRSS2-ERG fusion in a large series of PCa cases. Materials and Methods: Four hundred twenty-seven radical retropubic prostatectomy tissue samples were used to construct 10 tissue microarrays (TMAs). FISH analysis was previously conducted using dual-color interphase break-apart probes for the 5′ and 3′ regions of the ERG gene. ERG expression was evaluated using a commercial rabbit anti-ERG monoclonal antibody (clone EPR3864; Epitomics, Burlingame, CA). Each TMA spot was independently assessed, and any nuclear staining positivity was considered as indicative of ERG expression. Results: TMPRSS2-ERG fusions were detected by FISH in 195 (45.7%) of the PCa cases. ERG immunoexpression was found in 192 (45.0%) of the PCa cases and in none of the nontumoral tissue samples. Mean ERG H-scores were significantly higher in tumors harboring FISH-detected TMPRSS2-ERG fusions (P

Original languageEnglish (US)
Pages (from-to)1014-1020
Number of pages7
JournalAmerican Journal of Surgical Pathology
Volume35
Issue number7
DOIs
StatePublished - Jul 2011

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Fluorescence In Situ Hybridization
Adenocarcinoma
Immunohistochemistry
Interphase
Prostatectomy
Reverse Transcription
Clone Cells
Color
Monoclonal Antibodies
Staining and Labeling
Rabbits
Polymerase Chain Reaction
Genes
Neoplasms

Keywords

  • Edel
  • ERG expression
  • Esplit
  • fluorescence in situ hybridization
  • prostate adenocarcinoma
  • TMPRSS2-ERG fusion

ASJC Scopus subject areas

  • Anatomy
  • Pathology and Forensic Medicine
  • Surgery

Cite this

Immunohistochemistry for ERG expression as a surrogate for TMPRSS2-ERG fusion detection in prostatic adenocarcinomas. / Chaux, Alcides; Albadine, Roula; Toubaji, Antoun; Hicks, Jessica; Meeker, Alan Keith; Platz, Elizabeth A; Demarzo, Angelo Michael; Netto, George J.

In: American Journal of Surgical Pathology, Vol. 35, No. 7, 07.2011, p. 1014-1020.

Research output: Contribution to journalArticle

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