Immunohistochemical studies of keratin in human bronchus and lung tumors

Thomas Scott Wilson, E. M. McDowell, B. F. Trump

Research output: Contribution to journalArticle

Abstract

Lung tumors of various types, fixed in 4% formaldehyde-1% glutaraldehyde, were stained for keratin proteins. The results were compared with previous ultrastructural evidence of intermediate filament bundles (IFBs), presumed to be keratin. Electron and light microscopic methods were largely complimentary and the results were in agreement in 79% of cases. Light microscopy was superior for demonstrating keratinizing foci containing numerous well-developed IFBs, whereas electron microscopy was superior when keratin filaments were sparsely distributed in cells throughout a tumor. Fetal and adult bronchial specimens were also studied. Normal adult bronchus, fixed in aldehydes, was unreactive but keratin was observed in similarly fixed bronchi that showed epidermoid metaplasia and/or intraepithelial carcinoma. Keratin was demonstrated in normal adult bronchi fixed in ethanol. Keratin was not observed in fetal lung until the 14th week of gestation, when it appeared in basal cells and a few columnar cells of the larger bronchi. Thereafter, keratin progressively appeared in the more distal branches. As specimens from gestations of less than 14 weeks were fixed in aldehydes, the apparent lack of immunoreactivity may have been artifactual. Nevertheless, keratin was demonstrable in aldehyde-fixed fetal bronchi at 16 and 23 weeks' gestation.

Original languageEnglish (US)
Pages (from-to)621-628
Number of pages8
JournalArchives of Pathology and Laboratory Medicine
Volume109
Issue number7
StatePublished - Jan 1 1985
Externally publishedYes

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Bronchi
Keratins
Lung
Neoplasms
Aldehydes
Intermediate Filaments
Pregnancy
Light
Carcinoma in Situ
Metaplasia
Glutaral
Formaldehyde
Microscopy
Squamous Cell Carcinoma
Electron Microscopy
Ethanol
Electrons

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Medical Laboratory Technology

Cite this

Immunohistochemical studies of keratin in human bronchus and lung tumors. / Wilson, Thomas Scott; McDowell, E. M.; Trump, B. F.

In: Archives of Pathology and Laboratory Medicine, Vol. 109, No. 7, 01.01.1985, p. 621-628.

Research output: Contribution to journalArticle

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