Immunohistochemical mapping of enkephalin containing cell bodies, fibers and nerve terminals in the brain stem of the rat

George R. Uhl, Robert R. Goodman, Michael J. Kuhar, Steven R. Childers, Solomon H Snyder

Research output: Contribution to journalArticle

Abstract

Enkephalin immunoreactive perikarya, fibers and nerve terminals, visualized by the indirect immunohistofluorescent method in colchicine-pretreated animals, are localized in many discrete regions of the rat brain stem. These specific immunohistofluorescent patterns are similar after staining with selective primary antisera directed against either methione-enkephalin or leucine-enkephalin. Cell bodies are found in the substantia gelatinosa and interpolaris zones of the trigeminal nuclear complex, the nucleus of the solitary tract, in the vicinity of the nucleus raphé magnus, in the dorsal cochlear, medial vestibular, and paraolivary nuclei and, dorsal to this last region, in the parabrachial nuclei and the dorsal tegmental nucleus of Gudden, in the periaqueductal gray matter and interpeduncular nucleus and along the borders of the lateral lemniscus and medial geniculate. In some areas, such as the parabrachial region, nucleus of the solitary tract and substantia gelatinosa of the trigeminal nucleus, these perikarya are associated with densities of fibers and terminals. Other regions, such as the dorsal cochlear nucleus and the vicinity of the nucleus raphé magnus, contain cell bodies associated with low densities of processes and terminals. In still other nuclei, such as the nucleus of the facial nerve and the locus coeruleus, fiber and terminal densities without associated cell bodies are evident. Many of these enkephalin localizations can be rationalized on the basis of known actions of opiate drugs and the brain stem distribution of opiate receptors.

Original languageEnglish (US)
Pages (from-to)75-94
Number of pages20
JournalBrain Research
Volume166
Issue number1
DOIs
StatePublished - Apr 20 1979

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Enkephalins
Substantia Gelatinosa
Nerve Fibers
Trigeminal Nuclei
Brain Stem
Solitary Nucleus
Opiate Alkaloids
Vestibular Nuclei
Cochlear Nucleus
Leucine Enkephalin
Periaqueductal Gray
Locus Coeruleus
Cochlea
Facial Nerve
Colchicine
Opioid Receptors
Immune Sera
Staining and Labeling
Pharmaceutical Preparations
Cell Body

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

Immunohistochemical mapping of enkephalin containing cell bodies, fibers and nerve terminals in the brain stem of the rat. / Uhl, George R.; Goodman, Robert R.; Kuhar, Michael J.; Childers, Steven R.; Snyder, Solomon H.

In: Brain Research, Vol. 166, No. 1, 20.04.1979, p. 75-94.

Research output: Contribution to journalArticle

Uhl, George R. ; Goodman, Robert R. ; Kuhar, Michael J. ; Childers, Steven R. ; Snyder, Solomon H. / Immunohistochemical mapping of enkephalin containing cell bodies, fibers and nerve terminals in the brain stem of the rat. In: Brain Research. 1979 ; Vol. 166, No. 1. pp. 75-94.
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