Immunohistochemical localization of inhibin-α in the placenta and gestational trophoblastic lesions

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Abstract

The immunohistochemical distribution of inhibin-α in formalin-fixed, paraffin-embedded tissues was evaluated in placentas (2 to 40 weeks of gestation), implantation sites, and a variety of trophoblastic lesions. In the first trimester placenta, inhibin-α was strongly and diffusely expressed in syncytiotrophoblast. Implantation site intermediate trophoblast in normal and exaggerated placental sites was either negative or only weakly and focally positive for inhibin-α. With increasing gestational age, the staining intensity and distribution of inhibin-α decreased in syncytiotrophoblast but increased in the implantation site intermediate trophoblast. Chorionic-type intermediate trophoblast, present in the chorion laeve of the term placenta, was weakly but diffusely positive for inhibin- α. Cytotrophoblast remained negative for inhibin-α throughout gestation. In trophoblastic lesions, inhibin-α immunoreactivity was detected in all 17 hydatidiform moles (7 complete and 10 partial), 32 placental site nodules, 23 placental site trophoblastic tumors, 15 epithelioid trophoblastic tumors, and 16 choriocarcinomas. Inhibin-α immunoreactivity was confined to the syncytiotrophoblast in hydatidiform moles and choriocarcinoma. As with the normal placenta, inhibin-α was not detected in cytotrophoblast. To evaluate the utility of inhibin-α in the differential diagnosis of gestational trophoblastic lesions, we tested 32 squamous cell carcinoma of the cervix, 11 low-grade endometrial stromal sarcomas, 12 endometrial (7 well differentiated and 5 moderately differentiated) carcinomas, 7 epithelioid leiomyomas, and 10 leiomyosarcomas for the expression of inhibin-α. None of these lesions was positive. These data indicate that inhibin-α is expressed by all populations of trophoblast except cytotrophoblast and in all gestational trophoblastic lesions. Accordingly, immunohistochemical detection of inhibin-α is useful in the differential diagnosis of gestational trophoblastic lesions.

Original languageEnglish (US)
Pages (from-to)144-150
Number of pages7
JournalInternational Journal of Gynecological Pathology
Volume18
Issue number2
StatePublished - Apr 1999

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Inhibins
Placenta
Trophoblasts
Hydatidiform Mole
Choriocarcinoma
Placental Site Trophoblastic Tumor
Differential Diagnosis
Epithelioid Leiomyoma
Endometrial Stromal Sarcoma
Trophoblastic Neoplasms
Chorion
Pregnancy
Leiomyosarcoma
First Pregnancy Trimester
Cervix Uteri
Paraffin
Formaldehyde
Gestational Age
Squamous Cell Carcinoma

Keywords

  • Choriocarcinoma
  • Gestational trophoblastic disease
  • Hydatidiform mole
  • Inhibin
  • Mel-CAM
  • Placenta
  • Placental site trophoblastic tumor
  • Trophoblast

ASJC Scopus subject areas

  • Pathology and Forensic Medicine
  • Obstetrics and Gynecology

Cite this

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title = "Immunohistochemical localization of inhibin-α in the placenta and gestational trophoblastic lesions",
abstract = "The immunohistochemical distribution of inhibin-α in formalin-fixed, paraffin-embedded tissues was evaluated in placentas (2 to 40 weeks of gestation), implantation sites, and a variety of trophoblastic lesions. In the first trimester placenta, inhibin-α was strongly and diffusely expressed in syncytiotrophoblast. Implantation site intermediate trophoblast in normal and exaggerated placental sites was either negative or only weakly and focally positive for inhibin-α. With increasing gestational age, the staining intensity and distribution of inhibin-α decreased in syncytiotrophoblast but increased in the implantation site intermediate trophoblast. Chorionic-type intermediate trophoblast, present in the chorion laeve of the term placenta, was weakly but diffusely positive for inhibin- α. Cytotrophoblast remained negative for inhibin-α throughout gestation. In trophoblastic lesions, inhibin-α immunoreactivity was detected in all 17 hydatidiform moles (7 complete and 10 partial), 32 placental site nodules, 23 placental site trophoblastic tumors, 15 epithelioid trophoblastic tumors, and 16 choriocarcinomas. Inhibin-α immunoreactivity was confined to the syncytiotrophoblast in hydatidiform moles and choriocarcinoma. As with the normal placenta, inhibin-α was not detected in cytotrophoblast. To evaluate the utility of inhibin-α in the differential diagnosis of gestational trophoblastic lesions, we tested 32 squamous cell carcinoma of the cervix, 11 low-grade endometrial stromal sarcomas, 12 endometrial (7 well differentiated and 5 moderately differentiated) carcinomas, 7 epithelioid leiomyomas, and 10 leiomyosarcomas for the expression of inhibin-α. None of these lesions was positive. These data indicate that inhibin-α is expressed by all populations of trophoblast except cytotrophoblast and in all gestational trophoblastic lesions. Accordingly, immunohistochemical detection of inhibin-α is useful in the differential diagnosis of gestational trophoblastic lesions.",
keywords = "Choriocarcinoma, Gestational trophoblastic disease, Hydatidiform mole, Inhibin, Mel-CAM, Placenta, Placental site trophoblastic tumor, Trophoblast",
author = "Shih, {Ie Ming} and Kurman, {Robert J}",
year = "1999",
month = "4",
language = "English (US)",
volume = "18",
pages = "144--150",
journal = "International Journal of Gynecological Pathology",
issn = "0277-1691",
publisher = "Lippincott Williams and Wilkins",
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T1 - Immunohistochemical localization of inhibin-α in the placenta and gestational trophoblastic lesions

AU - Shih, Ie Ming

AU - Kurman, Robert J

PY - 1999/4

Y1 - 1999/4

N2 - The immunohistochemical distribution of inhibin-α in formalin-fixed, paraffin-embedded tissues was evaluated in placentas (2 to 40 weeks of gestation), implantation sites, and a variety of trophoblastic lesions. In the first trimester placenta, inhibin-α was strongly and diffusely expressed in syncytiotrophoblast. Implantation site intermediate trophoblast in normal and exaggerated placental sites was either negative or only weakly and focally positive for inhibin-α. With increasing gestational age, the staining intensity and distribution of inhibin-α decreased in syncytiotrophoblast but increased in the implantation site intermediate trophoblast. Chorionic-type intermediate trophoblast, present in the chorion laeve of the term placenta, was weakly but diffusely positive for inhibin- α. Cytotrophoblast remained negative for inhibin-α throughout gestation. In trophoblastic lesions, inhibin-α immunoreactivity was detected in all 17 hydatidiform moles (7 complete and 10 partial), 32 placental site nodules, 23 placental site trophoblastic tumors, 15 epithelioid trophoblastic tumors, and 16 choriocarcinomas. Inhibin-α immunoreactivity was confined to the syncytiotrophoblast in hydatidiform moles and choriocarcinoma. As with the normal placenta, inhibin-α was not detected in cytotrophoblast. To evaluate the utility of inhibin-α in the differential diagnosis of gestational trophoblastic lesions, we tested 32 squamous cell carcinoma of the cervix, 11 low-grade endometrial stromal sarcomas, 12 endometrial (7 well differentiated and 5 moderately differentiated) carcinomas, 7 epithelioid leiomyomas, and 10 leiomyosarcomas for the expression of inhibin-α. None of these lesions was positive. These data indicate that inhibin-α is expressed by all populations of trophoblast except cytotrophoblast and in all gestational trophoblastic lesions. Accordingly, immunohistochemical detection of inhibin-α is useful in the differential diagnosis of gestational trophoblastic lesions.

AB - The immunohistochemical distribution of inhibin-α in formalin-fixed, paraffin-embedded tissues was evaluated in placentas (2 to 40 weeks of gestation), implantation sites, and a variety of trophoblastic lesions. In the first trimester placenta, inhibin-α was strongly and diffusely expressed in syncytiotrophoblast. Implantation site intermediate trophoblast in normal and exaggerated placental sites was either negative or only weakly and focally positive for inhibin-α. With increasing gestational age, the staining intensity and distribution of inhibin-α decreased in syncytiotrophoblast but increased in the implantation site intermediate trophoblast. Chorionic-type intermediate trophoblast, present in the chorion laeve of the term placenta, was weakly but diffusely positive for inhibin- α. Cytotrophoblast remained negative for inhibin-α throughout gestation. In trophoblastic lesions, inhibin-α immunoreactivity was detected in all 17 hydatidiform moles (7 complete and 10 partial), 32 placental site nodules, 23 placental site trophoblastic tumors, 15 epithelioid trophoblastic tumors, and 16 choriocarcinomas. Inhibin-α immunoreactivity was confined to the syncytiotrophoblast in hydatidiform moles and choriocarcinoma. As with the normal placenta, inhibin-α was not detected in cytotrophoblast. To evaluate the utility of inhibin-α in the differential diagnosis of gestational trophoblastic lesions, we tested 32 squamous cell carcinoma of the cervix, 11 low-grade endometrial stromal sarcomas, 12 endometrial (7 well differentiated and 5 moderately differentiated) carcinomas, 7 epithelioid leiomyomas, and 10 leiomyosarcomas for the expression of inhibin-α. None of these lesions was positive. These data indicate that inhibin-α is expressed by all populations of trophoblast except cytotrophoblast and in all gestational trophoblastic lesions. Accordingly, immunohistochemical detection of inhibin-α is useful in the differential diagnosis of gestational trophoblastic lesions.

KW - Choriocarcinoma

KW - Gestational trophoblastic disease

KW - Hydatidiform mole

KW - Inhibin

KW - Mel-CAM

KW - Placenta

KW - Placental site trophoblastic tumor

KW - Trophoblast

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