Immunohistochemical localization of histaminase (diamine oxidase) in decidual cells of human placenta

W. R. Weisburger, G. Mendelsohn, J. C. Eggleston, Stephen B Baylin

Research output: Contribution to journalArticle

Abstract

An immunohistochemical technique has been developed for the localization of histaminase (diamine oxidase) in human placenta. The procedure utilizes antibody to highly purified human placental enzyme prepared in rabbits. Histaminase is shown to be present in the cytoplasm of the decidual cells of the maternal placenta; the staining is specific and can be removed by preabsorption of the antisera with purified antigen. The present demonstration of histaminase in the maternal portion of placenta confirms previous biochemical data suggesting that the enzyme is not a fetal product in this tissue. This study also provides further evidence that the presence of histaminase in some human tumors and in normal tissues like kidney and intestine is the result of the expression of a mature and not a fetal genome.

Original languageEnglish (US)
Pages (from-to)703-706
Number of pages4
JournalLaboratory Investigation
Volume38
Issue number6
StatePublished - 1978

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Amine Oxidase (Copper-Containing)
Placenta
Mothers
Enzymes
Intestines
Immune Sera
Cytoplasm
Genome
Staining and Labeling
Rabbits
Kidney
Antigens
Antibodies
Neoplasms

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Immunohistochemical localization of histaminase (diamine oxidase) in decidual cells of human placenta. / Weisburger, W. R.; Mendelsohn, G.; Eggleston, J. C.; Baylin, Stephen B.

In: Laboratory Investigation, Vol. 38, No. 6, 1978, p. 703-706.

Research output: Contribution to journalArticle

Weisburger, W. R. ; Mendelsohn, G. ; Eggleston, J. C. ; Baylin, Stephen B. / Immunohistochemical localization of histaminase (diamine oxidase) in decidual cells of human placenta. In: Laboratory Investigation. 1978 ; Vol. 38, No. 6. pp. 703-706.
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