TY - JOUR
T1 - Immunohistochemical insights into sickle cell retinopathy
AU - Lutty, Gerard A.
AU - Merges, Carol
AU - Crone, Sylvia
AU - Mcleod, D. Scott
N1 - Funding Information:
The authors acknowledge the Cincinnati Eye Bank, the Medical Eye Bank of Maryland, and the Centralized Pathology Unit for Sickle Cell Disease (Mobile, AL) for providing the tissue used in this study. We also acknowledge Dr. Donald Rucknagel from the Cincinnati Sickle Cell Center, and Drs. Elizabeth Manci and Don Culberson from the Centralized Pathology Unit for Sickle Cell Disease, Mobile, AL, for providing histories on the sickle cell subjects. We would also like to thank Drs. Kathy Flanders and Michael Sporn (N.C.I., N.I.H.) and Dr. Ira Herman (Tufts Medical Center, Boston, MA) for providing antibodies, and Drs. S. Charles Selden and Robert Prendergast for their critical review of this manuscript. This work was funded by the Titus Foundation, an unrestricted grant from Research to Prevent Blindness, Inc. (Wilmer Institute), and N.I.H. grants HL45922 (GL) and EY01765 (Wilmer Institute).
PY - 1994
Y1 - 1994
N2 - Dynamic vaso-occlusive and vaso-proliferative events occur in sickle cell retinopathy. Using streptavidin peroxidase immuno-histochemistry, we investigated changes in distribution and relative levels of components in the fibrinolytic system and growth factors in retina and choroid from 2 sickle cell patients: a 20 month old SS patient and a 54 year old SC patient. Antigen localization in the sickle cell patients was compared to localization from 2 non-sickle cell, non-diabetic control subjects. In chorioretinal pigmented lesions of the SC patient, bFGF and TGF-β1, β2, and β3 immunoreactivity was present within migrating retinal pigment epithelial (RPE) cells. Our interpretation of the data presented in this case study is that fibrin deposition within retinal and choroidal vessels of sickle cell subjects may occur due to elevated PAI-1 activity. Moreover, vaso-occlusions of choroidal vessels may influence the expression of growth factors in choriocapillaris endothelium, which could stimulate formation of choroidal neovascularization. Finally, fibrosis and gliosis in and near chorioretinal pigmented lesions may be stimulated by RPE production of bFGF and TGF-βs.
AB - Dynamic vaso-occlusive and vaso-proliferative events occur in sickle cell retinopathy. Using streptavidin peroxidase immuno-histochemistry, we investigated changes in distribution and relative levels of components in the fibrinolytic system and growth factors in retina and choroid from 2 sickle cell patients: a 20 month old SS patient and a 54 year old SC patient. Antigen localization in the sickle cell patients was compared to localization from 2 non-sickle cell, non-diabetic control subjects. In chorioretinal pigmented lesions of the SC patient, bFGF and TGF-β1, β2, and β3 immunoreactivity was present within migrating retinal pigment epithelial (RPE) cells. Our interpretation of the data presented in this case study is that fibrin deposition within retinal and choroidal vessels of sickle cell subjects may occur due to elevated PAI-1 activity. Moreover, vaso-occlusions of choroidal vessels may influence the expression of growth factors in choriocapillaris endothelium, which could stimulate formation of choroidal neovascularization. Finally, fibrosis and gliosis in and near chorioretinal pigmented lesions may be stimulated by RPE production of bFGF and TGF-βs.
KW - Basic fibroblast growth factor
KW - Fibrinolysis
KW - Human
KW - Ocular neovascularization
KW - Retinal pigment epithelial cells
KW - Sickle cell retinopathy
KW - Transforming growth factor-β
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U2 - 10.3109/02713689409042407
DO - 10.3109/02713689409042407
M3 - Article
C2 - 8194359
AN - SCOPUS:0028282159
SN - 0271-3683
VL - 13
SP - 125
EP - 138
JO - Current Eye Research
JF - Current Eye Research
IS - 2
ER -