Immunohistochemical evaluation of TMPRSS2-ERG gene fusion in adenosis of the prostate

Whitney M. Green, Jessica L. Hicks, Angela De Marzo, Peter P.B. Illei, Jonathan I. Epstein

Research output: Contribution to journalArticle

Abstract

Adenosis (atypical adenomatous hyperplasia) is a benign lesion that morphologically mimics prostate adenocarcinoma, although the relationship between these 2 lesions is still debated. The TMPRSS2-ERG fusion is a common chromosomal rearrangement that occurs early in the development of invasive adenocarcinoma of the prostate and results in the expression of a truncated ERG protein. This fusion is present in 50% of adenocarcinomas and in 20% of high-grade prostate intraepithelial lesions. Until recently, fluorescent in situ hybridization was the only method available to detect these rearrangements. A specific anti-ERG antibody is now available for detecting ERG protein expression and serves as a useful marker for ERG rearrangements. Formalin-fixed, paraffin-embedded tissue sections of adenosis from cases of prostate biopsies (n = 30), transurethral resections of the prostate (n = 12), and radical prostatectomies (n = 3) were analyzed via immunohistochemistry for ERG. None (0%) of the foci of adenosis were positive for ERG protein expression. Of 40 cases of Gleason score 6 adenocarcinoma on a tissue microarray, 22 (55%) were positive for ERG protein. Of the positive cases, 14 (63.6%) were moderate in intensity, with the remaining 36.4% being weak. The lack of ERG expression in adenosis supports the notion that it is not a precursor lesion of adenocarcinoma. Moreover, it suggests that immunohistochemistry for ERG expression could be a useful tool to differentiate adenosis from adenocarcinoma.

Original languageEnglish (US)
Pages (from-to)1895-1901
Number of pages7
JournalHuman pathology
Volume44
Issue number9
DOIs
StatePublished - Sep 1 2013

Keywords

  • Adenocarcinoma
  • Adenosis
  • ERG
  • Prostate
  • TMPRSS2

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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