Immunohistochemical detection of chlamydial antigens in association with cystitis

M. S. Shurbaji, J. S. Dumler, W. R. Gage, G. L. Pettis, P. K. Gupta, F. P. Kuhadja

Research output: Contribution to journalArticle

Abstract

To investigate the etiologic role of Chlamydia trachomatis in cystitis, the authors used the immunoperoxidase technique with a monoclonal antibody against Chlamydia and examined paraffin sections from 36 cases of histologically proven cystitis. The average patients' age was 60 (range, 2-85) years. Biopsies were taken for follow-up of treated bladder carcinoma (19), hematuria (8), and other nonneoplastic conditions (9). Chlamydial antigens were detected by immunohistochemistry in 12 (33%) of these 36 cases. Staining for Chlamydia occurred in the upper layers of the transitional epithelium and involved long stretches of epithelium. Underlying inflammation was usually chronic but did not have specific distinguishing features. Eight of the Chlamydia-positive biopsies were taken for follow-up of treated carcinoma, two were for hematuria, one for neurogenic bladder, and one for evaluation of sterile pyuria. Eleven (92%) of these 12 positive cases had a history of recent urologic instrumentation, in contrast to only 11 (46%) of 24 negative cases (P <0.02). There was no significant difference in the age or sex distribution between the two groups. The authors conclude that Chlamydia trachomatis can ascend the urethra and infect the bladder urothelium. Urologic instrumentation enhances the ability of Chlamydia to reach the bladder. Chlamydia trachomatis may play an etiologic role in cystitis.

Original languageEnglish (US)
Pages (from-to)363-366
Number of pages4
JournalAmerican Journal of Clinical Pathology
Volume93
Issue number3
StatePublished - 1990

Fingerprint

Cystitis
Chlamydia
Chlamydia trachomatis
Antigens
Urinary Bladder
Hematuria
Epithelium
Pyuria
Carcinoma
Biopsy
Urothelium
Neurogenic Urinary Bladder
Sex Distribution
Age Distribution
Urethra
Immunoenzyme Techniques
Paraffin
Immunohistochemistry
Monoclonal Antibodies
Staining and Labeling

Keywords

  • Chlamydia trachomatis
  • cystitis
  • immunoperoxidase technique
  • monoclonal antibody

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Shurbaji, M. S., Dumler, J. S., Gage, W. R., Pettis, G. L., Gupta, P. K., & Kuhadja, F. P. (1990). Immunohistochemical detection of chlamydial antigens in association with cystitis. American Journal of Clinical Pathology, 93(3), 363-366.

Immunohistochemical detection of chlamydial antigens in association with cystitis. / Shurbaji, M. S.; Dumler, J. S.; Gage, W. R.; Pettis, G. L.; Gupta, P. K.; Kuhadja, F. P.

In: American Journal of Clinical Pathology, Vol. 93, No. 3, 1990, p. 363-366.

Research output: Contribution to journalArticle

Shurbaji, MS, Dumler, JS, Gage, WR, Pettis, GL, Gupta, PK & Kuhadja, FP 1990, 'Immunohistochemical detection of chlamydial antigens in association with cystitis', American Journal of Clinical Pathology, vol. 93, no. 3, pp. 363-366.
Shurbaji MS, Dumler JS, Gage WR, Pettis GL, Gupta PK, Kuhadja FP. Immunohistochemical detection of chlamydial antigens in association with cystitis. American Journal of Clinical Pathology. 1990;93(3):363-366.
Shurbaji, M. S. ; Dumler, J. S. ; Gage, W. R. ; Pettis, G. L. ; Gupta, P. K. ; Kuhadja, F. P. / Immunohistochemical detection of chlamydial antigens in association with cystitis. In: American Journal of Clinical Pathology. 1990 ; Vol. 93, No. 3. pp. 363-366.
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