Immunohistochemical analysis of tumor polyamines discriminates high-risk patients undergoing nephrectomy for renal cell carcinoma

Nathalie Rioux-Leclercq, Jean Guy Delcros, Jean Yves Bansard, Jonathan Ira Epstein, Jean Jacques Patard, Bernard Lobel, François Guillé, Florence Jouan, Pascale Bellaud, Jacques Philippe Moulinoux

Research output: Contribution to journalArticle

Abstract

Renal cell carcinoma (RCC) is known to display a wide variation in biological behavior and clinical outcome. Although usual bioclinical prognostic parameters (eg, nuclear grade, tumor stage) are to a certain extent useful in predicting the outcome of RCC after radical nephrectomy, they now appear to be insufficient. The polyamines (spermidine, spermine, and putrescine) are ubiquitous polycations that are essential for cell proliferation. To support their excessive proliferation, cancer cells have high rates of polyamine metabolism. Indeed, malignant cells typically have higher polyamine levels than their normal counterparts. Before this report, antipolyamine antibodies that are potentially valuable tools for the in situ observation of polyamines had not been exploited in clinical conditions. In the present study, tumor tissues obtained from radical nephrectomy performed for RCC (n = 73) were immunostained with the anti-spermine monoclonal antibody Spm8-2, and the immunoreactivity was evaluated as a prognostic tool. RCC cells displayed various reactivity to the antibody Spm8-2 that translated into a heterogeneous cytoplasmic staining. The prognostic value of the labeling index (LI) on clinical outcome was correlated with the usual clinicopathologic parameters, and the cell proliferation rate was evaluated using Ki-67 labeling. Multiple correspondence analysis and ascending hierarchical classification were performed to determine significant prognostic factors. Univariate statistical survival analysis demonstrated that tumor size (P <.001), nuclear grade (P <.01), necrosis (P <.007), tumor stage (P <.004), metastasis (P <.001), Ki-67 LI (P <.0003), and Spm8-2 immunoreactivity (P <.0001) were predictors of tumor-related death. A positive correlation was found between Ki-67 LI and Spm8-2 immunoreactivity (r′ = .53). Multivariate analysis revealed that only Ki-67 LI and Spm8-2 immunoreactivity were significant independent factors in patients with metastases (P <.04 and

Original languageEnglish (US)
Pages (from-to)1279-1284
Number of pages6
JournalHuman Pathology
Volume35
Issue number10
DOIs
StatePublished - Oct 2004

Fingerprint

Polyamines
Nephrectomy
Renal Cell Carcinoma
Neoplasms
Spermine
Cell Proliferation
Neoplasm Metastasis
Putrescine
Spermidine
Antibodies
Survival Analysis
Necrosis
Multivariate Analysis
Monoclonal Antibodies
Observation
Staining and Labeling

Keywords

  • AHC
  • ascending hierarchical classification
  • CI
  • confidence interval
  • Ki-67
  • labeling index
  • LI
  • MCA
  • multiple correspondence analysis
  • PBS
  • phosphate-buffered saline
  • polyamines
  • prognosis
  • RCC
  • renal cell carcinoma

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Immunohistochemical analysis of tumor polyamines discriminates high-risk patients undergoing nephrectomy for renal cell carcinoma. / Rioux-Leclercq, Nathalie; Delcros, Jean Guy; Bansard, Jean Yves; Epstein, Jonathan Ira; Patard, Jean Jacques; Lobel, Bernard; Guillé, François; Jouan, Florence; Bellaud, Pascale; Moulinoux, Jacques Philippe.

In: Human Pathology, Vol. 35, No. 10, 10.2004, p. 1279-1284.

Research output: Contribution to journalArticle

Rioux-Leclercq, N, Delcros, JG, Bansard, JY, Epstein, JI, Patard, JJ, Lobel, B, Guillé, F, Jouan, F, Bellaud, P & Moulinoux, JP 2004, 'Immunohistochemical analysis of tumor polyamines discriminates high-risk patients undergoing nephrectomy for renal cell carcinoma', Human Pathology, vol. 35, no. 10, pp. 1279-1284. https://doi.org/10.1016/j.humpath.2004.07.002
Rioux-Leclercq, Nathalie ; Delcros, Jean Guy ; Bansard, Jean Yves ; Epstein, Jonathan Ira ; Patard, Jean Jacques ; Lobel, Bernard ; Guillé, François ; Jouan, Florence ; Bellaud, Pascale ; Moulinoux, Jacques Philippe. / Immunohistochemical analysis of tumor polyamines discriminates high-risk patients undergoing nephrectomy for renal cell carcinoma. In: Human Pathology. 2004 ; Vol. 35, No. 10. pp. 1279-1284.
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abstract = "Renal cell carcinoma (RCC) is known to display a wide variation in biological behavior and clinical outcome. Although usual bioclinical prognostic parameters (eg, nuclear grade, tumor stage) are to a certain extent useful in predicting the outcome of RCC after radical nephrectomy, they now appear to be insufficient. The polyamines (spermidine, spermine, and putrescine) are ubiquitous polycations that are essential for cell proliferation. To support their excessive proliferation, cancer cells have high rates of polyamine metabolism. Indeed, malignant cells typically have higher polyamine levels than their normal counterparts. Before this report, antipolyamine antibodies that are potentially valuable tools for the in situ observation of polyamines had not been exploited in clinical conditions. In the present study, tumor tissues obtained from radical nephrectomy performed for RCC (n = 73) were immunostained with the anti-spermine monoclonal antibody Spm8-2, and the immunoreactivity was evaluated as a prognostic tool. RCC cells displayed various reactivity to the antibody Spm8-2 that translated into a heterogeneous cytoplasmic staining. The prognostic value of the labeling index (LI) on clinical outcome was correlated with the usual clinicopathologic parameters, and the cell proliferation rate was evaluated using Ki-67 labeling. Multiple correspondence analysis and ascending hierarchical classification were performed to determine significant prognostic factors. Univariate statistical survival analysis demonstrated that tumor size (P <.001), nuclear grade (P <.01), necrosis (P <.007), tumor stage (P <.004), metastasis (P <.001), Ki-67 LI (P <.0003), and Spm8-2 immunoreactivity (P <.0001) were predictors of tumor-related death. A positive correlation was found between Ki-67 LI and Spm8-2 immunoreactivity (r′ = .53). Multivariate analysis revealed that only Ki-67 LI and Spm8-2 immunoreactivity were significant independent factors in patients with metastases (P <.04 and",
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AU - Bansard, Jean Yves

AU - Epstein, Jonathan Ira

AU - Patard, Jean Jacques

AU - Lobel, Bernard

AU - Guillé, François

AU - Jouan, Florence

AU - Bellaud, Pascale

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AB - Renal cell carcinoma (RCC) is known to display a wide variation in biological behavior and clinical outcome. Although usual bioclinical prognostic parameters (eg, nuclear grade, tumor stage) are to a certain extent useful in predicting the outcome of RCC after radical nephrectomy, they now appear to be insufficient. The polyamines (spermidine, spermine, and putrescine) are ubiquitous polycations that are essential for cell proliferation. To support their excessive proliferation, cancer cells have high rates of polyamine metabolism. Indeed, malignant cells typically have higher polyamine levels than their normal counterparts. Before this report, antipolyamine antibodies that are potentially valuable tools for the in situ observation of polyamines had not been exploited in clinical conditions. In the present study, tumor tissues obtained from radical nephrectomy performed for RCC (n = 73) were immunostained with the anti-spermine monoclonal antibody Spm8-2, and the immunoreactivity was evaluated as a prognostic tool. RCC cells displayed various reactivity to the antibody Spm8-2 that translated into a heterogeneous cytoplasmic staining. The prognostic value of the labeling index (LI) on clinical outcome was correlated with the usual clinicopathologic parameters, and the cell proliferation rate was evaluated using Ki-67 labeling. Multiple correspondence analysis and ascending hierarchical classification were performed to determine significant prognostic factors. Univariate statistical survival analysis demonstrated that tumor size (P <.001), nuclear grade (P <.01), necrosis (P <.007), tumor stage (P <.004), metastasis (P <.001), Ki-67 LI (P <.0003), and Spm8-2 immunoreactivity (P <.0001) were predictors of tumor-related death. A positive correlation was found between Ki-67 LI and Spm8-2 immunoreactivity (r′ = .53). Multivariate analysis revealed that only Ki-67 LI and Spm8-2 immunoreactivity were significant independent factors in patients with metastases (P <.04 and

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