Immunohistochemical analysis of NF-κB in human tumor tissue

Clint T. Allen, Carter van Waes

Research output: Contribution to journalArticlepeer-review

Abstract

Immunohistochemistry is a valuable molecular technique based upon the principle of antibody specifi city for target antigens in tissues with subsequent development of an amplifi ed colorimetric signal. When staining specifi city is ensured with the use of an isotype control, it allows for semiquantitative comparisons, tissue/ cellular localization, and inference regarding activation status of proteins of interest. Here we describe a protocol for immunohistochemical analysis of NF-κB family members in fresh frozen human tumor samples.

Original languageEnglish (US)
Pages (from-to)459-468
Number of pages10
JournalMethods in Molecular Biology
Volume1280
DOIs
StatePublished - 2015

Keywords

  • Frozen tissue
  • Histoscore
  • Immunohistochemistry
  • Isotype control
  • NF-κB
  • Peroxidase
  • Tissue fi xation

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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