TY - JOUR
T1 - Immunohistochemical analysis of intrathyroidal lymphocytes in Graves' disease
T2 - Evidence of activated T cells and production of interferon-γ
AU - Margolick, Joseph B.
AU - Weetman, Anthony P.
AU - Burman, Kenneth D.
N1 - Funding Information:
We thank Dr. Juan d’Avis for surgical assistance, Drs. C. V. Benton, A. Bargellesi, T. Waldmann, and S. F. Schlossman for gifts of monoclonal antibodies, Dr. Anthony S. Fauci for helpful discussions, and Ms. Beverly Taylor for preparation of the manuscript. This work was supported in part by a grant from the Mellon Foundation for faculty development, an institutional grant from the American Cancer Society (to Johns Hopkins University), BRSG Grant 12-86 from Johns Hopkins University, and Grant ES 03819 from the National Institutes of Health. Dr. Weetman is supported by the Wellcome Trust.
PY - 1988/5
Y1 - 1988/5
N2 - Previous immunohistochemical studies of the thyroid gland in Graves' disease (GD) have demonstrated that (i) lymphoid cells infiltrating the interstitium of the gland predominantly express the CD4+ (T-helper) phenotype; (ii) lymphoid cells infiltrating the epithelial compartment express predominantly the CD8+ antigen; and (iii) thyroid epithelial cells (thyrocytes) express class II histocompatibility antigens, possibly in response to endogenous production of interferon-γ (IFN-γ) by the infiltrating lymphocytes. In the present study the infiltrating lymphoid cells and thyrocytes in GD were further characterized by immunocytochemistry. Scattered infiltrating interstitial lymphocytes stained positively with antibodies directed against IFN-γ and the receptor for interleukin 2. These lymphocytes were most prominent around the periphery of lymphoid nodules. The interstitial lymphocytes predominantly expressed the CD4+ 2H4+ phenotype rather than the complementary CD4+ 4B4+ phenotype, suggesting the presence of the suppressor-inducer rather than helper-inducer T cell subset. The infiltrating lymphocytes were associated with thyrocytes expressing HLA-DR antigens, as previously reported, and also HLA-DQ antigens, but to a lesser extent. The presence of HLA-DQ may also suggest the possibility of an induced suppressor response. The intraepithelial CD8+ cells did not stain with antibodies to CD3, 2H4, 4B4, or Leu 7 and their identity remains unknown.
AB - Previous immunohistochemical studies of the thyroid gland in Graves' disease (GD) have demonstrated that (i) lymphoid cells infiltrating the interstitium of the gland predominantly express the CD4+ (T-helper) phenotype; (ii) lymphoid cells infiltrating the epithelial compartment express predominantly the CD8+ antigen; and (iii) thyroid epithelial cells (thyrocytes) express class II histocompatibility antigens, possibly in response to endogenous production of interferon-γ (IFN-γ) by the infiltrating lymphocytes. In the present study the infiltrating lymphoid cells and thyrocytes in GD were further characterized by immunocytochemistry. Scattered infiltrating interstitial lymphocytes stained positively with antibodies directed against IFN-γ and the receptor for interleukin 2. These lymphocytes were most prominent around the periphery of lymphoid nodules. The interstitial lymphocytes predominantly expressed the CD4+ 2H4+ phenotype rather than the complementary CD4+ 4B4+ phenotype, suggesting the presence of the suppressor-inducer rather than helper-inducer T cell subset. The infiltrating lymphocytes were associated with thyrocytes expressing HLA-DR antigens, as previously reported, and also HLA-DQ antigens, but to a lesser extent. The presence of HLA-DQ may also suggest the possibility of an induced suppressor response. The intraepithelial CD8+ cells did not stain with antibodies to CD3, 2H4, 4B4, or Leu 7 and their identity remains unknown.
UR - http://www.scopus.com/inward/record.url?scp=0023927988&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0023927988&partnerID=8YFLogxK
U2 - 10.1016/0090-1229(88)90073-6
DO - 10.1016/0090-1229(88)90073-6
M3 - Article
C2 - 2964967
AN - SCOPUS:0023927988
SN - 0090-1229
VL - 47
SP - 208
EP - 218
JO - Clinical Immunology and Immunopathology
JF - Clinical Immunology and Immunopathology
IS - 2
ER -