Immunohistochemical analysis of differentiation in static and mixed prostate cancer spheroids

Hong Song, Kim C. O'Connor, Odile David, Carrie L. Giordano, Helena Pappas-LeBeau, Sanda Clejan

Research output: Contribution to journalArticle

Abstract

Neoplastic multicellular spheroids are in vitro models of solid tumors employed in drug testing and basic research. This study compares differentiation in static and mixed prostate cancer spheroids. Staining intensity of prostate specific antigen (PSA) was down-regulated upon mixing, from 0.21 ± 0.03 to 0.13 ± 0.03 in LNCaP multicellular spheroids, and from 0.13 ± 0.04 to 0.03 ± 0.02 in DU 145 multicellular spheroids. This was accompanied by 65% increase in the expression of cytokeratins 8 and 18 in DU 145 spheroids. PSA expression extended 60 μm within static spheroids and was disrupted in mixed culture. Diminished PSA expression and spatial organization suggests a more aggressive cancer. Higher cytokeratin expression could result from either differentiation towards a luminal phenotype or activation of the Ras pathway during dedifferentiation. Thus, the existing paradigm of differentiation established for normal tissue does not apply for our neoplastic spheroids. Cell dedifferentiation is attributed to improved interstitial transport and synthesis of extracellular matrix.

Original languageEnglish (US)
Pages (from-to)180-186
Number of pages7
JournalJournal of Cellular and Molecular Medicine
Volume7
Issue number2
StatePublished - Apr 2003
Externally publishedYes

Fingerprint

Cellular Spheroids
Prostate-Specific Antigen
Prostatic Neoplasms
Cell Dedifferentiation
Keratin-8
Keratin-18
Keratins
Extracellular Matrix
Tumors
Neoplasms
Chemical activation
Tissue
Staining and Labeling
Phenotype
Testing
Research
Pharmaceutical Preparations

Keywords

  • Cytokeratins
  • Differentiation
  • Mixing
  • Prostate cancer
  • PSA
  • Spheroid

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry

Cite this

Song, H., O'Connor, K. C., David, O., Giordano, C. L., Pappas-LeBeau, H., & Clejan, S. (2003). Immunohistochemical analysis of differentiation in static and mixed prostate cancer spheroids. Journal of Cellular and Molecular Medicine, 7(2), 180-186.

Immunohistochemical analysis of differentiation in static and mixed prostate cancer spheroids. / Song, Hong; O'Connor, Kim C.; David, Odile; Giordano, Carrie L.; Pappas-LeBeau, Helena; Clejan, Sanda.

In: Journal of Cellular and Molecular Medicine, Vol. 7, No. 2, 04.2003, p. 180-186.

Research output: Contribution to journalArticle

Song, H, O'Connor, KC, David, O, Giordano, CL, Pappas-LeBeau, H & Clejan, S 2003, 'Immunohistochemical analysis of differentiation in static and mixed prostate cancer spheroids', Journal of Cellular and Molecular Medicine, vol. 7, no. 2, pp. 180-186.
Song H, O'Connor KC, David O, Giordano CL, Pappas-LeBeau H, Clejan S. Immunohistochemical analysis of differentiation in static and mixed prostate cancer spheroids. Journal of Cellular and Molecular Medicine. 2003 Apr;7(2):180-186.
Song, Hong ; O'Connor, Kim C. ; David, Odile ; Giordano, Carrie L. ; Pappas-LeBeau, Helena ; Clejan, Sanda. / Immunohistochemical analysis of differentiation in static and mixed prostate cancer spheroids. In: Journal of Cellular and Molecular Medicine. 2003 ; Vol. 7, No. 2. pp. 180-186.
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