Abstract
Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing abl(ts). This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline V(H) segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the pre-B cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region.
Original language | English (US) |
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Pages (from-to) | 611-618 |
Number of pages | 8 |
Journal | EMBO Journal |
Volume | 11 |
Issue number | 2 |
DOIs | |
State | Published - 1992 |
Keywords
- A-MuLV transformed cells
- Circular DNA
- Temperature-sensitive abl
- V-VDJ joining
ASJC Scopus subject areas
- Neuroscience(all)
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)
- Immunology and Microbiology(all)