Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism

Sadakazu Usuda, Toshitada Takemori, Masao Matsuoka, Takuji Shirasawa, Kazuya Yoshida, Akio Mori, Kimishige Ishizaka, Hitoshi Sakano

Research output: Contribution to journalArticle

Abstract

Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing ablts. This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline VH segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the preB cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region.

Original languageEnglish (US)
Pages (from-to)611-618
Number of pages8
JournalThe EMBO journal
Volume11
Issue number2
StatePublished - 1992
Externally publishedYes

Fingerprint

Immunoglobulin Variable Region
Immunoglobulin Genes
Circular DNA
Genes
Genetic Recombination
DNA
Protein Sorting Signals
Cells
Immunoglobulin Heavy Chain Genes
Cell Line
Transformed Cell Line
Immunoglobulin Heavy Chains
Lymphocytes
Immunoglobulins
Temperature

Keywords

  • A-MuLV transformed cells
  • Circular DNA
  • Temperature-sensitive abl
  • V -VDJ joining

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Usuda, S., Takemori, T., Matsuoka, M., Shirasawa, T., Yoshida, K., Mori, A., ... Sakano, H. (1992). Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism. The EMBO journal, 11(2), 611-618.

Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism. / Usuda, Sadakazu; Takemori, Toshitada; Matsuoka, Masao; Shirasawa, Takuji; Yoshida, Kazuya; Mori, Akio; Ishizaka, Kimishige; Sakano, Hitoshi.

In: The EMBO journal, Vol. 11, No. 2, 1992, p. 611-618.

Research output: Contribution to journalArticle

Usuda, S, Takemori, T, Matsuoka, M, Shirasawa, T, Yoshida, K, Mori, A, Ishizaka, K & Sakano, H 1992, 'Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism', The EMBO journal, vol. 11, no. 2, pp. 611-618.
Usuda S, Takemori T, Matsuoka M, Shirasawa T, Yoshida K, Mori A et al. Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism. The EMBO journal. 1992;11(2):611-618.
Usuda, Sadakazu ; Takemori, Toshitada ; Matsuoka, Masao ; Shirasawa, Takuji ; Yoshida, Kazuya ; Mori, Akio ; Ishizaka, Kimishige ; Sakano, Hitoshi. / Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism. In: The EMBO journal. 1992 ; Vol. 11, No. 2. pp. 611-618.
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