Abstract
Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing ablts. This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline VH segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the preB cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 611-618 |
| Number of pages | 8 |
| Journal | The EMBO journal |
| Volume | 11 |
| Issue number | 2 |
| State | Published - 1992 |
| Externally published | Yes |
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Keywords
- A-MuLV transformed cells
- Circular DNA
- Temperature-sensitive abl
- V -VDJ joining
ASJC Scopus subject areas
- Genetics
- Cell Biology
Cite this
Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism. / Usuda, Sadakazu; Takemori, Toshitada; Matsuoka, Masao; Shirasawa, Takuji; Yoshida, Kazuya; Mori, Akio; Ishizaka, Kimishige; Sakano, Hitoshi.
In: The EMBO journal, Vol. 11, No. 2, 1992, p. 611-618.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism
AU - Usuda, Sadakazu
AU - Takemori, Toshitada
AU - Matsuoka, Masao
AU - Shirasawa, Takuji
AU - Yoshida, Kazuya
AU - Mori, Akio
AU - Ishizaka, Kimishige
AU - Sakano, Hitoshi
PY - 1992
Y1 - 1992
N2 - Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing ablts. This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline VH segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the preB cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region.
AB - Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing ablts. This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline VH segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the preB cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region.
KW - A-MuLV transformed cells
KW - Circular DNA
KW - Temperature-sensitive abl
KW - V -VDJ joining
UR - http://www.scopus.com/inward/record.url?scp=0026554998&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0026554998&partnerID=8YFLogxK
M3 - Article
C2 - 1311252
AN - SCOPUS:0026554998
VL - 11
SP - 611
EP - 618
JO - EMBO Journal
JF - EMBO Journal
SN - 0261-4189
IS - 2
ER -