Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism

S. Usuda, T. Takemori, M. Matsuoka, T. Shirasawa, K. Yoshida, A. Mori, K. Ishizaka, H. Sakano

Research output: Contribution to journalArticlepeer-review

Abstract

Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing abl(ts). This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline V(H) segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the pre-B cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region.

Original languageEnglish (US)
Pages (from-to)611-618
Number of pages8
JournalEMBO Journal
Volume11
Issue number2
DOIs
StatePublished - 1992

Keywords

  • A-MuLV transformed cells
  • Circular DNA
  • Temperature-sensitive abl
  • V-VDJ joining

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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