Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism

S. Usuda; T. Takemori; M. Matsuoka; T. Shirasawa; K. Yoshida; A. Mori; K. Ishizaka; H. Sakano

doi:10.1002/j.1460-2075.1992.tb05093.x

Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism

S. Usuda, T. Takemori, M. Matsuoka, T. Shirasawa, K. Yoshida, A. Mori, K. Ishizaka, H. Sakano

Research output: Contribution to journal › Article › peer-review

Abstract

Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing abl(ts). This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline V(H) segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the pre-B cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region.

Original language	English (US)
Pages (from-to)	611-618
Number of pages	8
Journal	EMBO Journal
Volume	11
Issue number	2
DOIs	https://doi.org/10.1002/j.1460-2075.1992.tb05093.x
State	Published - 1992
Externally published	Yes

Keywords

A-MuLV transformed cells
Circular DNA
Temperature-sensitive abl
V-VDJ joining

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Biochemistry, Genetics and Molecular Biology(all)
Immunology and Microbiology(all)

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10.1002/j.1460-2075.1992.tb05093.x

Cite this

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title = "Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism",

abstract = "Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing abl(ts). This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline V(H) segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the pre-B cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region.",

keywords = "A-MuLV transformed cells, Circular DNA, Temperature-sensitive abl, V-VDJ joining",

author = "S. Usuda and T. Takemori and M. Matsuoka and T. Shirasawa and K. Yoshida and A. Mori and K. Ishizaka and H. Sakano",

year = "1992",

doi = "10.1002/j.1460-2075.1992.tb05093.x",

language = "English (US)",

volume = "11",

pages = "611--618",

journal = "EMBO Journal",

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publisher = "Nature Publishing Group",

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T1 - Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism

AU - Usuda, S.

AU - Takemori, T.

AU - Matsuoka, M.

AU - Shirasawa, T.

AU - Yoshida, K.

AU - Mori, A.

AU - Ishizaka, K.

AU - Sakano, H.

PY - 1992

Y1 - 1992

N2 - Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing abl(ts). This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline V(H) segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the pre-B cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region.

AB - Circular DNA resulting from V gene replacement was studied with an A-MuLV transformed cell line containing abl(ts). This cell line undergoes V gene replacement at elevated temperatures in the immunoglobulin (Ig) heavy chain (H) gene. Examination of circular DNA revealed that a heptamer-related sequence (TACTGTG) within the coding region of VDJ was joined to the recombination signal sequence (RSS) of a germline V(H) segment. This provides direct evidence for a intramolecular DNA deletion mechanism for V gene replacement. In the pre-B cell line as well as in in vivo lymphocytes, unusual circular DNAs were found which were structurally similar to the V gene replacement circles. They represented excision products of the deletion type recombination between one complete RSS and a heptamer-like sequence in the Ig H region.

KW - A-MuLV transformed cells

KW - Circular DNA

KW - Temperature-sensitive abl

KW - V-VDJ joining

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Immunoglobulin V gene replacement is caused by the intramolecular DNA deletion mechanism

Abstract

Keywords

ASJC Scopus subject areas

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