Immunoglobulin switch circular DNA in the mouse infected with Nippostrongylus brasiliensis: Evidence for successive class switching from μ to ε via γ

Kazuya Yoshida, Masao Matsuoka, Sadakazu Usuda, Akio Mori, Kimishige Ishizaka, Hitoshi Sakano

Research output: Contribution to journalArticle

Abstract

We have characterized immunoglobulin switch circular DNA in mice infected with the nematode parasite Nippostrongylus brasiliensis. Two kinds of circular DNA were identified in the lymph nodes as excision products of switch recombination of immunoglobulin heavy-chain constant region (CH) genes. One is a recombinant between Cμ and Cγ1 (γ1 circle), and the other is a recombinant between Cγ1 and Cε (ε circle). In the ε circle, a short piece of switch μ (Sμ) sequence was inserted between Sε and Sγ1 sequences. The inserted Sμ sequence could be a trace of the preceding switch from Cμ to Cγ1. These findings indicate that parasitic infection can induce class switch recombinations in a successive manner, first from Cμ to Cγ1, and then from Cγ1 to Cε.

Original languageEnglish (US)
Pages (from-to)7829-7833
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number20
StatePublished - 1990
Externally publishedYes

Fingerprint

Nippostrongylus
Immunoglobulin Class Switching
Circular DNA
Genetic Recombination
Immunoglobulins
Immunoglobulin Heavy Chains
Parasitic Diseases
Lymph Node Excision
Parasites
Genes

Keywords

  • IgE
  • IgG1
  • Interleukin 4
  • Parasite infection
  • Switch recombination

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Immunoglobulin switch circular DNA in the mouse infected with Nippostrongylus brasiliensis : Evidence for successive class switching from μ to ε via γ. / Yoshida, Kazuya; Matsuoka, Masao; Usuda, Sadakazu; Mori, Akio; Ishizaka, Kimishige; Sakano, Hitoshi.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 87, No. 20, 1990, p. 7829-7833.

Research output: Contribution to journalArticle

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