Immunoglobulin superfamily members play an important role in the mosquito immune system

Lindsey S. Garver; Zhiyong Xi; George Dimopoulos

doi:10.1016/j.dci.2007.09.007

Immunoglobulin superfamily members play an important role in the mosquito immune system

Lindsey S. Garver, Zhiyong Xi, George Dimopoulos

Bloomberg School of Public Health

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

Immunoglobulin superfamily (IgSF) proteins are known for their ability to specifically recognize and adhere to other molecules, mediating cell-surface reception and pathogen recognition. Mammalian IgSF proteins such as antibodies are among the best characterized molecules of the immune system; in contrast, the involvement of invertebrate IgSF members in immunity has not been broadly studied. Analysis of the predicted Anopheles gambiae transcriptome identified 138 proteins that have at least one immunoglobulin domain. Challenge with Plasmodium, Gram-negative or Gram-positive bacteria resulted in significant regulation of 85 IgSF genes, indicating potential roles for these molecules in infection responses and immunity. Based on sequence and expression data, six infection-responsive with immunoglobulin domain (IRID 1-6) genes were chosen and functionally characterized with regard to their role in innate immunity. Reverse-genetic gene-silencing assays showed IRID3, IRID5 and IRID6 contribute to viability upon bacterial infection while IRID4 and IRID6 are involved in limiting Plasmodium falciparum infection.

Original language	English (US)
Pages (from-to)	519-531
Number of pages	13
Journal	Developmental and Comparative Immunology
Volume	32
Issue number	5
DOIs	https://doi.org/10.1016/j.dci.2007.09.007
State	Published - 2008

Keywords

Anopheles gambiae
IgSF
Immunoglobulin domain
Innate immunity
Malaria
Plasmodium

ASJC Scopus subject areas

Immunology
Developmental Biology

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10.1016/j.dci.2007.09.007

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title = "Immunoglobulin superfamily members play an important role in the mosquito immune system",

abstract = "Immunoglobulin superfamily (IgSF) proteins are known for their ability to specifically recognize and adhere to other molecules, mediating cell-surface reception and pathogen recognition. Mammalian IgSF proteins such as antibodies are among the best characterized molecules of the immune system; in contrast, the involvement of invertebrate IgSF members in immunity has not been broadly studied. Analysis of the predicted Anopheles gambiae transcriptome identified 138 proteins that have at least one immunoglobulin domain. Challenge with Plasmodium, Gram-negative or Gram-positive bacteria resulted in significant regulation of 85 IgSF genes, indicating potential roles for these molecules in infection responses and immunity. Based on sequence and expression data, six infection-responsive with immunoglobulin domain (IRID 1-6) genes were chosen and functionally characterized with regard to their role in innate immunity. Reverse-genetic gene-silencing assays showed IRID3, IRID5 and IRID6 contribute to viability upon bacterial infection while IRID4 and IRID6 are involved in limiting Plasmodium falciparum infection.",

keywords = "Anopheles gambiae, IgSF, Immunoglobulin domain, Innate immunity, Malaria, Plasmodium",

author = "Garver, {Lindsey S.} and Zhiyong Xi and George Dimopoulos",

note = "Funding Information: We are grateful to Dr. Yuemei Dong for sharing gene expression data. We thank the microarray core facility, the parasitology core facility and the insectary personnel at the Johns Hopkins Malaria Research Institute for assistance with microarray, P. falciparum culture and mosquito rearing. We also thank Dr. Deborah McClellan at the Editing Referral Service, William H. Welch Medical Library, Johns Hopkins University School of Medicine. This material is based upon work supported under a National Science Foundation Graduate Research Fellowship (L.G.). This work is also supported by the National Institutes of Health/National Institute for Allergy and Infectious Disease 1R01AI061576-01A1, United Nations Development Program/World Bank/World Health Organization Special Program for Research and Training in Tropical Diseases, The Ellison Medical Foundation, Johns Hopkins School of Public Health and Johns Hopkins Malaria Research Institute. ",

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AU - Garver, Lindsey S.

AU - Xi, Zhiyong

AU - Dimopoulos, George

N1 - Funding Information: We are grateful to Dr. Yuemei Dong for sharing gene expression data. We thank the microarray core facility, the parasitology core facility and the insectary personnel at the Johns Hopkins Malaria Research Institute for assistance with microarray, P. falciparum culture and mosquito rearing. We also thank Dr. Deborah McClellan at the Editing Referral Service, William H. Welch Medical Library, Johns Hopkins University School of Medicine. This material is based upon work supported under a National Science Foundation Graduate Research Fellowship (L.G.). This work is also supported by the National Institutes of Health/National Institute for Allergy and Infectious Disease 1R01AI061576-01A1, United Nations Development Program/World Bank/World Health Organization Special Program for Research and Training in Tropical Diseases, The Ellison Medical Foundation, Johns Hopkins School of Public Health and Johns Hopkins Malaria Research Institute.

PY - 2008

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N2 - Immunoglobulin superfamily (IgSF) proteins are known for their ability to specifically recognize and adhere to other molecules, mediating cell-surface reception and pathogen recognition. Mammalian IgSF proteins such as antibodies are among the best characterized molecules of the immune system; in contrast, the involvement of invertebrate IgSF members in immunity has not been broadly studied. Analysis of the predicted Anopheles gambiae transcriptome identified 138 proteins that have at least one immunoglobulin domain. Challenge with Plasmodium, Gram-negative or Gram-positive bacteria resulted in significant regulation of 85 IgSF genes, indicating potential roles for these molecules in infection responses and immunity. Based on sequence and expression data, six infection-responsive with immunoglobulin domain (IRID 1-6) genes were chosen and functionally characterized with regard to their role in innate immunity. Reverse-genetic gene-silencing assays showed IRID3, IRID5 and IRID6 contribute to viability upon bacterial infection while IRID4 and IRID6 are involved in limiting Plasmodium falciparum infection.

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Immunoglobulin superfamily members play an important role in the mosquito immune system

Abstract

Keywords

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