Immunoglobulin E-mediated release of a kininogenase from purified human lung mast cells

D. Proud, D. W. MacGlashan, H. H. Newball, E. S. Schulman, L. M. Lichtenstein

Research output: Contribution to journalArticle

Abstract

Certain manifestations of the allergic response, such as vasodilation and edema, may be attributed to the involvement of kinins, but, as yet, little data exist to show how these peptides could be produced during IgE-mediated events. We now report that purified human lung mast cells contain a kininogenase that is released in a dose-dependent fashion by anti-IgE. Release of kinin-generating activity parallels that of histamine and, like histamine release, kininogenase release is temperature-dependent. Kininogenase release also parallels increasing histamine release when mast cells of increasing size are stimulated with an optimal level of anti-IgE. Finally, when mast cells of >99% purity were optimally challenged, kininogenase activity again paralleled histamine levels in the release supernatant and residual cell pellet. The mast cell kininogenase appears to be a preformed mediator in that it occurs in lysates of unstimulated mast cells of >99% purity. Optimal generation of kinin from highly purified human low molecular weight kininogen occurred at pH 5.5 and in 8 preparations was 380 ± 237 ng kinin/h/106 mast cells (X̄ ± SD). Thus, human lung mast cells contain a kininogenase that is released during, and may participate in, IgE-mediated inflammatory disorders in humans.

Original languageEnglish (US)
Pages (from-to)405-408
Number of pages4
JournalAmerican Review of Respiratory Disease
Volume132
Issue number2
StatePublished - Nov 28 1985

ASJC Scopus subject areas

  • Pulmonary and Respiratory Medicine

Fingerprint Dive into the research topics of 'Immunoglobulin E-mediated release of a kininogenase from purified human lung mast cells'. Together they form a unique fingerprint.

  • Cite this