Immunogenicity studies of guayule and guayule latex in occupationally exposed workers

Robert G Hamilton, Katrina Cornish

Research output: Contribution to journalArticle

Abstract

Type I Hevea brasiliensis rubber latex allergy is managed by avoidance, using synthetic and alternative latex (such as Parthenium argentatum, guayule) products. This study investigates the ability of high-dose occupational exposure to guayule shrub/homogenate/latex to induce guayule-specific antibody responses in employees (Yulex Corporation). Participants completed an allergy history/guayule exposure questionnaire and provided annual blood samples from 2006 to 2008. Sera were analyzed for IgG and IgE anti-guayule (protein from homogenate, commercial-grade latex and non-ammoniated total plant proteins) using solid phase immunoassays (negative = IgG <1 μg/ml, IgE <1 ng/ml). Guayule-specific IgG antibody (range: 2.0-9.7 μg/ml) was detected in 3 of 16 (19%) highly exposed employees in the pilot plant and R&D/applications laboratory. Antibody levels related to relative cumulative-years (e.g. >3) of reported guayule homogenate/latex exposure. Equivocal IgG antibody responses (1.0-2.0 μg/ml) were detected in 2 of 5 (40%) of administrators with infrequent guayule homogenate/latex contact. No guayule-specific IgE antibody or guayule-associated allergic reactions were detected. We conclude that protein from guayule and in guayule latex can be immunogenic but not allergenic in occupationally exposed workers.

Original languageEnglish (US)
Pages (from-to)197-201
Number of pages5
JournalIndustrial Crops and Products
Volume31
Issue number1
DOIs
StatePublished - Jan 2010

Fingerprint

guayule
latex
immune response
hypersensitivity
antibodies
Parthenium argentatum
occupational exposure
Hevea brasiliensis
corporations
plant proteins
blood serum
rubber
immunoassays
human resources
questionnaires
shrubs
proteins

Keywords

  • Guayule
  • Hevea brasiliensis
  • IgE antibody
  • IgG antibody
  • Latex allergy
  • Occupational exposure
  • Parthenium argentatum

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Immunogenicity studies of guayule and guayule latex in occupationally exposed workers. / Hamilton, Robert G; Cornish, Katrina.

In: Industrial Crops and Products, Vol. 31, No. 1, 01.2010, p. 197-201.

Research output: Contribution to journalArticle

@article{f30d630b9dc64d19abbf1ba23be3b354,
title = "Immunogenicity studies of guayule and guayule latex in occupationally exposed workers",
abstract = "Type I Hevea brasiliensis rubber latex allergy is managed by avoidance, using synthetic and alternative latex (such as Parthenium argentatum, guayule) products. This study investigates the ability of high-dose occupational exposure to guayule shrub/homogenate/latex to induce guayule-specific antibody responses in employees (Yulex Corporation). Participants completed an allergy history/guayule exposure questionnaire and provided annual blood samples from 2006 to 2008. Sera were analyzed for IgG and IgE anti-guayule (protein from homogenate, commercial-grade latex and non-ammoniated total plant proteins) using solid phase immunoassays (negative = IgG <1 μg/ml, IgE <1 ng/ml). Guayule-specific IgG antibody (range: 2.0-9.7 μg/ml) was detected in 3 of 16 (19{\%}) highly exposed employees in the pilot plant and R&D/applications laboratory. Antibody levels related to relative cumulative-years (e.g. >3) of reported guayule homogenate/latex exposure. Equivocal IgG antibody responses (1.0-2.0 μg/ml) were detected in 2 of 5 (40{\%}) of administrators with infrequent guayule homogenate/latex contact. No guayule-specific IgE antibody or guayule-associated allergic reactions were detected. We conclude that protein from guayule and in guayule latex can be immunogenic but not allergenic in occupationally exposed workers.",
keywords = "Guayule, Hevea brasiliensis, IgE antibody, IgG antibody, Latex allergy, Occupational exposure, Parthenium argentatum",
author = "Hamilton, {Robert G} and Katrina Cornish",
year = "2010",
month = "1",
doi = "10.1016/j.indcrop.2009.09.012",
language = "English (US)",
volume = "31",
pages = "197--201",
journal = "Industrial Crops and Products",
issn = "0926-6690",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - Immunogenicity studies of guayule and guayule latex in occupationally exposed workers

AU - Hamilton, Robert G

AU - Cornish, Katrina

PY - 2010/1

Y1 - 2010/1

N2 - Type I Hevea brasiliensis rubber latex allergy is managed by avoidance, using synthetic and alternative latex (such as Parthenium argentatum, guayule) products. This study investigates the ability of high-dose occupational exposure to guayule shrub/homogenate/latex to induce guayule-specific antibody responses in employees (Yulex Corporation). Participants completed an allergy history/guayule exposure questionnaire and provided annual blood samples from 2006 to 2008. Sera were analyzed for IgG and IgE anti-guayule (protein from homogenate, commercial-grade latex and non-ammoniated total plant proteins) using solid phase immunoassays (negative = IgG <1 μg/ml, IgE <1 ng/ml). Guayule-specific IgG antibody (range: 2.0-9.7 μg/ml) was detected in 3 of 16 (19%) highly exposed employees in the pilot plant and R&D/applications laboratory. Antibody levels related to relative cumulative-years (e.g. >3) of reported guayule homogenate/latex exposure. Equivocal IgG antibody responses (1.0-2.0 μg/ml) were detected in 2 of 5 (40%) of administrators with infrequent guayule homogenate/latex contact. No guayule-specific IgE antibody or guayule-associated allergic reactions were detected. We conclude that protein from guayule and in guayule latex can be immunogenic but not allergenic in occupationally exposed workers.

AB - Type I Hevea brasiliensis rubber latex allergy is managed by avoidance, using synthetic and alternative latex (such as Parthenium argentatum, guayule) products. This study investigates the ability of high-dose occupational exposure to guayule shrub/homogenate/latex to induce guayule-specific antibody responses in employees (Yulex Corporation). Participants completed an allergy history/guayule exposure questionnaire and provided annual blood samples from 2006 to 2008. Sera were analyzed for IgG and IgE anti-guayule (protein from homogenate, commercial-grade latex and non-ammoniated total plant proteins) using solid phase immunoassays (negative = IgG <1 μg/ml, IgE <1 ng/ml). Guayule-specific IgG antibody (range: 2.0-9.7 μg/ml) was detected in 3 of 16 (19%) highly exposed employees in the pilot plant and R&D/applications laboratory. Antibody levels related to relative cumulative-years (e.g. >3) of reported guayule homogenate/latex exposure. Equivocal IgG antibody responses (1.0-2.0 μg/ml) were detected in 2 of 5 (40%) of administrators with infrequent guayule homogenate/latex contact. No guayule-specific IgE antibody or guayule-associated allergic reactions were detected. We conclude that protein from guayule and in guayule latex can be immunogenic but not allergenic in occupationally exposed workers.

KW - Guayule

KW - Hevea brasiliensis

KW - IgE antibody

KW - IgG antibody

KW - Latex allergy

KW - Occupational exposure

KW - Parthenium argentatum

UR - http://www.scopus.com/inward/record.url?scp=71649107995&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=71649107995&partnerID=8YFLogxK

U2 - 10.1016/j.indcrop.2009.09.012

DO - 10.1016/j.indcrop.2009.09.012

M3 - Article

AN - SCOPUS:71649107995

VL - 31

SP - 197

EP - 201

JO - Industrial Crops and Products

JF - Industrial Crops and Products

SN - 0926-6690

IS - 1

ER -