Immunogenicity of recombinant Babesia microti hsp70 homologue in mice

Erdal Erol, N. Kumar, C. A. Carson

Research output: Contribution to journalArticle

Abstract

A Babesia microti hsp70 homologue was cloned, expressed in a prokaryotic system and tested in a pilot study for protection against lethal challenge. Results showed that 30% of the mice inoculated with recombinant protein (rBm hsp70) survived challenge, while all of the controls died. Evidence of antibody response to the hsp homologue was detected by Western blot analysis, but no protection was imparted through passive transfer of convalescent serum. Passively transferred spleen cells, from mice which survived challenge, also failed to impart protection. The mechanism of partial immunity suggested by these observations remains to be elucidated. Copyright (C) 1998 Elsevier Science B.V.

Original languageEnglish (US)
Pages (from-to)263-266
Number of pages4
JournalInternational Journal for Parasitology
Volume29
Issue number2
DOIs
Publication statusPublished - Feb 1 1999

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Keywords

  • Babesia microti
  • hsp70
  • Immunogenicity
  • Mouse
  • Vaccination

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases

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