Immunofluorescent study of histone H5 in chick erythroid cells from developing embryos and adults

Casilda Mura; P. C. Huang; Susan W. Craig

doi:10.1016/0047-6374(78)90057-X

Immunofluorescent study of histone H5 in chick erythroid cells from developing embryos and adults

Casilda Mura, P. C. Huang, Susan W. Craig

School of Medicine

Research output: Contribution to journal › Article › peer-review

10 Scopus citations

Abstract

Indirect immunofluorescence has been used to detect the presence of histone H5 in single cells during avian erythropoiesis. Evidence is presented which demonstrates that this method specifically detects histone H5. The results show that the increase in the amount of H5 which can be extracted from nuclei during erythropoiesis is accounted for both by an increase in the amount of H5 per cell and by an increase in the total number of cells containing histone H5. Of particular interest are the observations that histone H5 is arranged within the nucleus in several distinct, reproducible patterns and that different patterns predominate at different stages of erythroid cell development. In addition to suggesting new possibilities for the role of histone H5, these findings indicate that immunofluorescence may be used profitably to explore the supramolecular organization of chromatin.

Original language	English (US)
Pages (from-to)	109-122
Number of pages	14
Journal	Mechanisms of Ageing and Development
Volume	7
Issue number	C
DOIs	https://doi.org/10.1016/0047-6374(78)90057-X
State	Published - 1978

ASJC Scopus subject areas

Aging
Developmental Biology

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10.1016/0047-6374(78)90057-X

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@article{b24696043122427192478136cf5d3ad6,

title = "Immunofluorescent study of histone H5 in chick erythroid cells from developing embryos and adults",

abstract = "Indirect immunofluorescence has been used to detect the presence of histone H5 in single cells during avian erythropoiesis. Evidence is presented which demonstrates that this method specifically detects histone H5. The results show that the increase in the amount of H5 which can be extracted from nuclei during erythropoiesis is accounted for both by an increase in the amount of H5 per cell and by an increase in the total number of cells containing histone H5. Of particular interest are the observations that histone H5 is arranged within the nucleus in several distinct, reproducible patterns and that different patterns predominate at different stages of erythroid cell development. In addition to suggesting new possibilities for the role of histone H5, these findings indicate that immunofluorescence may be used profitably to explore the supramolecular organization of chromatin.",

author = "Casilda Mura and Huang, {P. C.} and Craig, {Susan W.}",

note = "Funding Information: This study was supported by grants from the Jane Coffin Childs Fund (S.W.C.) and the National Foundation/March of Dimes (P.C.H.).",

year = "1978",

doi = "10.1016/0047-6374(78)90057-X",

language = "English (US)",

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pages = "109--122",

journal = "Mechanisms of Ageing and Development",

issn = "0047-6374",

publisher = "Elsevier Ireland Ltd",

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TY - JOUR

T1 - Immunofluorescent study of histone H5 in chick erythroid cells from developing embryos and adults

AU - Mura, Casilda

AU - Huang, P. C.

AU - Craig, Susan W.

N1 - Funding Information: This study was supported by grants from the Jane Coffin Childs Fund (S.W.C.) and the National Foundation/March of Dimes (P.C.H.).

PY - 1978

Y1 - 1978

N2 - Indirect immunofluorescence has been used to detect the presence of histone H5 in single cells during avian erythropoiesis. Evidence is presented which demonstrates that this method specifically detects histone H5. The results show that the increase in the amount of H5 which can be extracted from nuclei during erythropoiesis is accounted for both by an increase in the amount of H5 per cell and by an increase in the total number of cells containing histone H5. Of particular interest are the observations that histone H5 is arranged within the nucleus in several distinct, reproducible patterns and that different patterns predominate at different stages of erythroid cell development. In addition to suggesting new possibilities for the role of histone H5, these findings indicate that immunofluorescence may be used profitably to explore the supramolecular organization of chromatin.

AB - Indirect immunofluorescence has been used to detect the presence of histone H5 in single cells during avian erythropoiesis. Evidence is presented which demonstrates that this method specifically detects histone H5. The results show that the increase in the amount of H5 which can be extracted from nuclei during erythropoiesis is accounted for both by an increase in the amount of H5 per cell and by an increase in the total number of cells containing histone H5. Of particular interest are the observations that histone H5 is arranged within the nucleus in several distinct, reproducible patterns and that different patterns predominate at different stages of erythroid cell development. In addition to suggesting new possibilities for the role of histone H5, these findings indicate that immunofluorescence may be used profitably to explore the supramolecular organization of chromatin.

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JO - Mechanisms of Ageing and Development

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Immunofluorescent study of histone H5 in chick erythroid cells from developing embryos and adults

Abstract

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