Immunofluorescent study of histone H5 in chick erythroid cells from developing embryos and adults

Casilda Mura, P. C. Huang, Susan W. Craig

Research output: Contribution to journalArticle

Abstract

Indirect immunofluorescence has been used to detect the presence of histone H5 in single cells during avian erythropoiesis. Evidence is presented which demonstrates that this method specifically detects histone H5. The results show that the increase in the amount of H5 which can be extracted from nuclei during erythropoiesis is accounted for both by an increase in the amount of H5 per cell and by an increase in the total number of cells containing histone H5. Of particular interest are the observations that histone H5 is arranged within the nucleus in several distinct, reproducible patterns and that different patterns predominate at different stages of erythroid cell development. In addition to suggesting new possibilities for the role of histone H5, these findings indicate that immunofluorescence may be used profitably to explore the supramolecular organization of chromatin.

Original languageEnglish (US)
Pages (from-to)109-122
Number of pages14
JournalMechanisms of Ageing and Development
Volume7
Issue numberC
DOIs
StatePublished - 1978

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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