Immunoenzyme assay for human prostatic acid phosphatase

B. K. Choe, N. R. Rose, M. Korol, E. J. Pontes

Research output: Contribution to journalArticle

Abstract

Human sera or plasma containing acid phosphatases were mixed with rabbit antiserum specific for prostatic acid phosphatase (PAP) and the resulting primary immune complexes were precipitated by ammonium sulfate. The amount of specifically precipitated PAP was estimated directly by its enzyme activity. Using this assay, as little as 10 ng/ml of the PAP could be determined. Excellent correlation of this assay method with other immunoassays of PAP was demonstrated.

Original languageEnglish (US)
Pages (from-to)396-400
Number of pages5
JournalExperimental Biology and Medicine
Volume162
Issue number3
StatePublished - 1979
Externally publishedYes

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Assays
Ammonium Sulfate
Enzyme activity
Acid Phosphatase
Antigen-Antibody Complex
Immunoassay
Immune Sera
Rabbits
Plasmas
prostatic acid phosphatase
Enzymes
Serum

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Choe, B. K., Rose, N. R., Korol, M., & Pontes, E. J. (1979). Immunoenzyme assay for human prostatic acid phosphatase. Experimental Biology and Medicine, 162(3), 396-400.

Immunoenzyme assay for human prostatic acid phosphatase. / Choe, B. K.; Rose, N. R.; Korol, M.; Pontes, E. J.

In: Experimental Biology and Medicine, Vol. 162, No. 3, 1979, p. 396-400.

Research output: Contribution to journalArticle

Choe, BK, Rose, NR, Korol, M & Pontes, EJ 1979, 'Immunoenzyme assay for human prostatic acid phosphatase', Experimental Biology and Medicine, vol. 162, no. 3, pp. 396-400.
Choe BK, Rose NR, Korol M, Pontes EJ. Immunoenzyme assay for human prostatic acid phosphatase. Experimental Biology and Medicine. 1979;162(3):396-400.
Choe, B. K. ; Rose, N. R. ; Korol, M. ; Pontes, E. J. / Immunoenzyme assay for human prostatic acid phosphatase. In: Experimental Biology and Medicine. 1979 ; Vol. 162, No. 3. pp. 396-400.
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