@inbook{7d6f8001c2724975add1902568586cec,
title = "Immunodetection of human LINE-1 expression in cultured cells and human tissues",
abstract = "Long interspersed element-1 (LINE-1) is the only active protein-coding retrotransposon in humans. It is not expressed in somatic tissue but is aberrantly expressed in a wide variety of human cancers. ORF1p protein is the most robust indicator of LINE-1 expression; the protein accumulates in large quantities in cellular cytoplasm. Recently, monoclonal antibodies have allowed more complete characterizations of ORF1p expression and indicated potential for developing ORF1p as a clinical biomarker. Here, we describe a mouse monoclonal antibody specifi c for human LINE-1 ORF1p and its application in immunofl uorescence and immunohistochemistry of both cells and human tissues. We also describe detection of tagged LINE-1 ORF2p via immunofl uorescence. These general methods may be readily adapted to use with many other proteins and antibodies.",
keywords = "Biomarker, Immunofl uorescence, Immunohistochemistry, LINE-1 ORF1p, P40, Tissue microarray, Tumor marker",
author = "Reema Sharma and Nemanja Rodi{\'c} and Burns, {Kathleen H.} and Taylor, {Martin S.}",
note = "Publisher Copyright: {\textcopyright} Springer Science+Business Media New York 2016.",
year = "2016",
doi = "10.1007/978-1-4939-3372-3_17",
language = "English (US)",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "261--280",
booktitle = "Methods in Molecular Biology",
}