Immunocytochemical evidence that the β-protein precursor is an integral component of neurofibrillary tangles of Alzheimer's disease

G. Perry, P. L. Richey, S. L. Siedlak, M. A. Smith, P. Mulvihill, D. A. DeWitt, J. Barnett, B. D. Greenberg, R. N. Kalaria

Research output: Contribution to journalArticlepeer-review

Abstract

Amyloid β (Aβ) immunoreactivity has been demonstrated in all extracellular neurofibrillary tangles (E-NFT) and most intraneuronal neurofibrillary tangles (I-NFT). We undertook this immunocytochemical study to understand the relationship between Aβ immunoreactivity localized in NFT and β-protein precursor (βPP). We found epitopes of amino-, mid-, and carboxyl-terminal domains of βPP in I-NFT and the majority of E-NFT. NFT retained βPP after ionic detergent extraction, demonstrating that βPP is an integral component of NFT. Finding βPP in regions of Aβ immunoreactivity raises the possibility that βPP or its fragments associate with amyloid, and that the stability of Aβ is responsible for its dominance in amyloid deposits.

Original languageEnglish (US)
Pages (from-to)1586-1593
Number of pages8
JournalAmerican Journal of Pathology
Volume143
Issue number6
StatePublished - Dec 1 1993
Externally publishedYes

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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