Immunocytochemical detection of lymphocyte surface antigens in fixed tissue sections

Michael J Borowitz, B. P. Croker, J. Burchette

Research output: Contribution to journalArticle

Abstract

Immunocytochemistry has been used extensively in the study of lymphomas on both fixed paraffin-embedded tissues and unfixed frozen tissue sections. The former technique, although preferable in terms of convenience and morphology, has been limited to the detection of cytoplasmic constituents (6), while surface markers, including surface immunoglobulin (SIg) and lymphocyte-associated antigens, have been reliably detected only on frozen sections of fresh tissue (1,2,4,8-10). Michel et al. (2) described a medium for transport of skin biopsies and demonstrated that frozen sections of tissue prepared in this fixative gave results comparable to freshly frozen tissue for immunofluorescent localization of immunoglobulin and complement deposits. We have used this technique for immunofluorescence of skin and kidney biopsies, and in this report evaluate the transport media for fixation of lymphocytes for immunohistochemical staining. We show that this fixative not only preserves the antigenicity of all surface lymphocyte antigens tested but also actually enhances reactivity of the T cell antigen and improves the localization of the antigens.

Original languageEnglish (US)
Pages (from-to)171-174
Number of pages4
JournalJournal of Histochemistry and Cytochemistry
Volume30
Issue number2
StatePublished - 1982
Externally publishedYes

Fingerprint

Surface Antigens
Lymphocytes
Frozen Sections
Fixatives
Biopsy
Antigens
B-Cell Antigen Receptors
Skin
Viral Tumor Antigens
Paraffin
Fluorescent Antibody Technique
Immunoglobulins
Lymphoma
Immunohistochemistry
Staining and Labeling
T-Lymphocytes
Kidney

ASJC Scopus subject areas

  • Cell Biology
  • Anatomy

Cite this

Immunocytochemical detection of lymphocyte surface antigens in fixed tissue sections. / Borowitz, Michael J; Croker, B. P.; Burchette, J.

In: Journal of Histochemistry and Cytochemistry, Vol. 30, No. 2, 1982, p. 171-174.

Research output: Contribution to journalArticle

@article{4006f329b9e3452eba2b414b6ada8617,
title = "Immunocytochemical detection of lymphocyte surface antigens in fixed tissue sections",
abstract = "Immunocytochemistry has been used extensively in the study of lymphomas on both fixed paraffin-embedded tissues and unfixed frozen tissue sections. The former technique, although preferable in terms of convenience and morphology, has been limited to the detection of cytoplasmic constituents (6), while surface markers, including surface immunoglobulin (SIg) and lymphocyte-associated antigens, have been reliably detected only on frozen sections of fresh tissue (1,2,4,8-10). Michel et al. (2) described a medium for transport of skin biopsies and demonstrated that frozen sections of tissue prepared in this fixative gave results comparable to freshly frozen tissue for immunofluorescent localization of immunoglobulin and complement deposits. We have used this technique for immunofluorescence of skin and kidney biopsies, and in this report evaluate the transport media for fixation of lymphocytes for immunohistochemical staining. We show that this fixative not only preserves the antigenicity of all surface lymphocyte antigens tested but also actually enhances reactivity of the T cell antigen and improves the localization of the antigens.",
author = "Borowitz, {Michael J} and Croker, {B. P.} and J. Burchette",
year = "1982",
language = "English (US)",
volume = "30",
pages = "171--174",
journal = "Journal of Histochemistry and Cytochemistry",
issn = "0022-1554",
publisher = "Histochemical Society Inc.",
number = "2",

}

TY - JOUR

T1 - Immunocytochemical detection of lymphocyte surface antigens in fixed tissue sections

AU - Borowitz, Michael J

AU - Croker, B. P.

AU - Burchette, J.

PY - 1982

Y1 - 1982

N2 - Immunocytochemistry has been used extensively in the study of lymphomas on both fixed paraffin-embedded tissues and unfixed frozen tissue sections. The former technique, although preferable in terms of convenience and morphology, has been limited to the detection of cytoplasmic constituents (6), while surface markers, including surface immunoglobulin (SIg) and lymphocyte-associated antigens, have been reliably detected only on frozen sections of fresh tissue (1,2,4,8-10). Michel et al. (2) described a medium for transport of skin biopsies and demonstrated that frozen sections of tissue prepared in this fixative gave results comparable to freshly frozen tissue for immunofluorescent localization of immunoglobulin and complement deposits. We have used this technique for immunofluorescence of skin and kidney biopsies, and in this report evaluate the transport media for fixation of lymphocytes for immunohistochemical staining. We show that this fixative not only preserves the antigenicity of all surface lymphocyte antigens tested but also actually enhances reactivity of the T cell antigen and improves the localization of the antigens.

AB - Immunocytochemistry has been used extensively in the study of lymphomas on both fixed paraffin-embedded tissues and unfixed frozen tissue sections. The former technique, although preferable in terms of convenience and morphology, has been limited to the detection of cytoplasmic constituents (6), while surface markers, including surface immunoglobulin (SIg) and lymphocyte-associated antigens, have been reliably detected only on frozen sections of fresh tissue (1,2,4,8-10). Michel et al. (2) described a medium for transport of skin biopsies and demonstrated that frozen sections of tissue prepared in this fixative gave results comparable to freshly frozen tissue for immunofluorescent localization of immunoglobulin and complement deposits. We have used this technique for immunofluorescence of skin and kidney biopsies, and in this report evaluate the transport media for fixation of lymphocytes for immunohistochemical staining. We show that this fixative not only preserves the antigenicity of all surface lymphocyte antigens tested but also actually enhances reactivity of the T cell antigen and improves the localization of the antigens.

UR - http://www.scopus.com/inward/record.url?scp=0020393433&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0020393433&partnerID=8YFLogxK

M3 - Article

C2 - 7037938

AN - SCOPUS:0020393433

VL - 30

SP - 171

EP - 174

JO - Journal of Histochemistry and Cytochemistry

JF - Journal of Histochemistry and Cytochemistry

SN - 0022-1554

IS - 2

ER -