Immunochemical properties of a polysaccharide antigen of Trichosporon beigelii that cross-reacts with the capsular glucuronoxylomannan of Cryptococcus neoformans

S. J.N. Devi, P. G. Reddy, C. A. Lyman, T. J. Walsh, C. E. Frasch, A. C. Bush

Research output: Contribution to journalArticlepeer-review

Abstract

Serum detection of a carbohydrate antigen of Trichosporon beigelii that cross-reacts with the capsular glucuronoxylomannan (GXM) of Cryptococcus neoformans is used as a diagnostic marker in disseminated trichosporonosis. We isolated and purified the cross-reactive polysaccharide antigen from Trichosporon beigelii, strain TCM. Immunochemical characterization of this carbohydrate antigen revealed that it shares antigenic determinant(s) with the capsular GXM of C. neoformans. It was a cell-associated, high molecular weight acidic polysaccharide which was released into the culture medium during growth in vitro. T. beigelii released 96-fold less polysaccharide into the culture supernatant than a clinical isolate of C. neoformans. Qualitative chemical analysis as determined by high-performance anion-exchange chromatography revealed that the polysaccharide was composed of mannose, xylose, glucose and glucuronic acid. Nuclear magnetic resonance spectroscopic analyses of native and modified polysaccharides suggested the presence of O-acetyl groups. The presence of O-acetyl and glucuronyl epitopes was confirmed serologically using epitope-specific antibodies. T. beigelii polysaccharide produced a precipitation line of partial identity with cryptococcal anti-GXM serum by immunodiffusion. Thus, the cell-associated carbohydrate antigen of T. beigelii is immunochemically similar to the GXM of C. neoformans and may play a role in pathogenesis.

Original languageEnglish (US)
Pages (from-to)87-92
Number of pages6
JournalImmunology and Infectious Diseases
Volume6
Issue number2
StatePublished - Dec 1 1996

Keywords

  • Cryptococcus neoformans
  • Glucuronoxylomannan
  • Immunochemistry
  • Polysaccharide
  • Trichosporon beigelii

ASJC Scopus subject areas

  • Immunology

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