Immunochemical detection of inducible NO synthase in human lung

W. R. Tracey, C. Xue, V. Klinghofer, J. Barlow, J. S. Pollock, U. Forstermann, R. A. Johns

Research output: Contribution to journalArticlepeer-review


Type II (inducible) nitric oxide synthase (NOS) may play an important role in pulmonary pathophysiology, yet it remains controversial whether human tissues are capable of expressing this protein. Therefore, a polyclonal antibody (8196) was raised against type II NOS from induced RAW 264.7 macrophages and used to investigate the expression of this enzyme in human lung tissue. Anti-type II NOS antibody did not cross-react with either neuronal (type I) or endothelial (type III) constitutive NOS, whereas a 130- kDa protein was detected in cytosol from induced macrophages or liver removed from lipopolysaccharide (25 mg/kg)-treated rats. Cells or tissues that lacked NOS activity did not express immunoreactive proteins. Similarly, in grossly normal human lung tissue, no immunoreactivity was detected with the anti- type II NOS antibody. In contrast, strong immunoreactivity was detected in alveolar macrophages present in lung tissue from a patient with bronchiectasis and acute bronchopneumonia. These data demonstrate that human alveolar macrophages are able to express type II NOS and support a role for this enzyme in pulmonary inflammatory pathophysiology.

Original languageEnglish (US)
Pages (from-to)L722-L727
JournalAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
Issue number6 10-6
StatePublished - 1994
Externally publishedYes


  • adult respiratory distress syndrome
  • bronchiectasis
  • chronic obstructive pulmonary disease
  • cytokine
  • inflammation
  • liver
  • macrophage
  • nitric oxide synthase
  • pneumonia
  • pulmonary

ASJC Scopus subject areas

  • Physiology
  • Pulmonary and Respiratory Medicine
  • Physiology (medical)
  • Cell Biology


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