TY - JOUR
T1 - Immunochemical characterization of surface antigens of TerC, a teratocarcinoma-derived cell line
AU - Larraga, V.
AU - Edidin, M.
PY - 1979
Y1 - 1979
N2 - Rabbit and mouse antisera prepared against teratocarcinoma cells precipitate both glycoproteins and glycolipids from detergent extracts of radiolabeled cells. Extracts of immunoprecipitates with chloroform/methanol, 2:1 (vol/vol) have been resolved on thin-layer gels into multiple peaks. There are more species seen in extracts of teratocarcinoma cells than in extracts of the crossreacting cultured cell line, cl 1d. The teratocarcinoma antigens may be extracted out of chloroform/methanol into buffered saline. Incubation in these secondary extracts converts unreactive cells (lymphocytes) to cells reactive with antisera against teratocarcinoma. Furthermore, the coated cells absorb at least 80% of the activity of antisera against teratocarcinoma targets.
AB - Rabbit and mouse antisera prepared against teratocarcinoma cells precipitate both glycoproteins and glycolipids from detergent extracts of radiolabeled cells. Extracts of immunoprecipitates with chloroform/methanol, 2:1 (vol/vol) have been resolved on thin-layer gels into multiple peaks. There are more species seen in extracts of teratocarcinoma cells than in extracts of the crossreacting cultured cell line, cl 1d. The teratocarcinoma antigens may be extracted out of chloroform/methanol into buffered saline. Incubation in these secondary extracts converts unreactive cells (lymphocytes) to cells reactive with antisera against teratocarcinoma. Furthermore, the coated cells absorb at least 80% of the activity of antisera against teratocarcinoma targets.
UR - http://www.scopus.com/inward/record.url?scp=0018745977&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0018745977&partnerID=8YFLogxK
U2 - 10.1073/pnas.76.6.2912
DO - 10.1073/pnas.76.6.2912
M3 - Article
C2 - 379868
AN - SCOPUS:0018745977
SN - 0027-8424
VL - 76
SP - 2912
EP - 2916
JO - Proceedings of the National Academy of Sciences of the United States of America
JF - Proceedings of the National Academy of Sciences of the United States of America
IS - 6
ER -