Immunochemical Characterization of a Human B Lymphocyte Differentiation Antigen (p65)

E. W. ADES, S. FERRONE, C. M. BALCH

Research output: Contribution to journalArticlepeer-review

Abstract

A human B‐cell differentiation antigen (BDA‐1) with a molecular weight of 65,000 was Identified by use of an anti‐B‐cell xenoantiserum. BDA‐1 was isolated by immunoprecipitation of cell lysates from a B‐lymphoblastoid cell line (SB) and from blood B lymphocytes (sIg+ER) of four normal individuals. It was not detectable in cell membrane lysates from two T‐lymphoblastoid cell lines (HSB‐2 and MOLT‐3) or from enriched normal T cells (sIgER+). BDA is a singlechain molecule since its migration in SDS‐PAGE gels was not altered by heating the protein to 100 C or by treatment with 2‐mercaptoetbanol. Immunodepletion experiments demonstrated that the antigen recognized by anti‐BDA xenoantiserum has neither structural nor antigenic relationships with la‐like antigen or β‐microglobulin.

Original languageEnglish (US)
Pages (from-to)519-523
Number of pages5
JournalScandinavian Journal of Immunology
Volume12
Issue number6
DOIs
StatePublished - Dec 1980
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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