A human B‐cell differentiation antigen (BDA‐1) with a molecular weight of 65,000 was Identified by use of an anti‐B‐cell xenoantiserum. BDA‐1 was isolated by immunoprecipitation of cell lysates from a B‐lymphoblastoid cell line (SB) and from blood B lymphocytes (sIg+ER−) of four normal individuals. It was not detectable in cell membrane lysates from two T‐lymphoblastoid cell lines (HSB‐2 and MOLT‐3) or from enriched normal T cells (sIg−ER+). BDA is a singlechain molecule since its migration in SDS‐PAGE gels was not altered by heating the protein to 100 C or by treatment with 2‐mercaptoetbanol. Immunodepletion experiments demonstrated that the antigen recognized by anti‐BDA xenoantiserum has neither structural nor antigenic relationships with la‐like antigen or β‐microglobulin.
|Original language||English (US)|
|Number of pages||5|
|Journal||Scandinavian Journal of Immunology|
|State||Published - Dec 1980|
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