Immunobead enzyme-linked immunosorbent assay for quantitating immunoglobulin A in human secretions amd serum

D. A. Sack, P. K.B. Neogi, M. K. Alam

Research output: Contribution to journalArticle

Abstract

We have developed an enzyme-linked immunosorbent assay for quantitating human immunoglobulin A in human secretions and serum. The procedure uses Immunobeads as the solid phase, has a sensitivity of 10 ng/ml, and can be completed in 1 day. Since there is no diffusion through agar, the assay does not require a correction factor when measuring secretory immunoglobulin A. This method should be useful in studies of local mucosal immunity in humans, especially when a very sensitive assay is needed.

Original languageEnglish (US)
Pages (from-to)281-283
Number of pages3
JournalInfection and immunity
Volume29
Issue number1
StatePublished - Jan 1 1980
Externally publishedYes

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Immunology
  • Infectious Diseases

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