We have developed an enzyme-linked immunosorbent assay for quantitating human immunoglobulin A in human secretions and serum. The procedure uses Immunobeads as the solid phase, has a sensitivity of 10 ng/ml, and can be completed in 1 day. Since there is no diffusion through agar, the assay does not require a correction factor when measuring secretory immunoglobulin A. This method should be useful in studies of local mucosal immunity in humans, especially when a very sensitive assay is needed.
|Original language||English (US)|
|Number of pages||3|
|Journal||Infection and immunity|
|State||Published - Jan 1 1980|
ASJC Scopus subject areas
- Infectious Diseases