Immunoaffinity Purification of Proteins

Jennifer M. Kavran, Daniel J. Leahy

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

This protocol entails a single-step, high-affinity purification of proteins using an immobilized antibody column.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages27-36
Number of pages10
DOIs
StatePublished - Jun 20 2015

Publication series

NameMethods in Enzymology
Volume559
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Binding buffer
  • Elution buffer
  • Immunoaffinity purification
  • Immunoaffinty columns
  • Neutralization buffer
  • SDS-PAGE

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Fingerprint Dive into the research topics of 'Immunoaffinity Purification of Proteins'. Together they form a unique fingerprint.

Cite this