Immunoaffinity-based biosensor for polycyclic aromatic hydrocarbons

C. B. Bargeron, R. C. Benson, T. E. Phillips, P. F. Scholl, S. Abubaker, J. D. Groopman, P. T. Strickland

Research output: Contribution to journalArticle

Abstract

An automated biosensor for 1-hydroxypyrene-glucuronide (OHPG) has been developed using a sensor platform initially designed for aflatoxin. The platform is based on the properties of immunoaffinity for sample purification and concentration, and fluorescence for detection. Experiments have demonstrated capture, wash, elution, and quantitation with very good results. The device is handheld and battery-driven, and can be miniaturized further. The bed volume of the column was 0.1 ml with a capacity of about 400 ng of the OHPG. Analysis time was 10-11 minutes. The sensitivity was about 0.5 ppb. The antibodies for the system were developed previously and recognize OHPG. The biosensor relies upon microprocessor-controlled minifluidics and fluorometry.

Original languageEnglish (US)
Pages (from-to)40-46
Number of pages7
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume4255
DOIs
StatePublished - Jan 1 2001

Keywords

  • Automated Biosensor
  • Fluorescence
  • Handheld
  • Hydroxypyrene
  • Hydroxypyrene-glucuronide
  • Immunoaffinity
  • Polycyclic aromatic hydrocarbon

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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