@article{2599d0901fa44a56ae785c6e76b4c198,
title = "Immunization with envelope subunit vaccine products elicits neutralizing antibodies against laboratory-adapted but not primary isolates of human immunodeficiency virus type 1",
abstract = "Phase I studies of volunteers not infected with human immunodeficiency virus type 1 (HIV-1) have shown that immunization with envelope subunit vaccine products elicits antibodies that neutralize laboratory-adapted (prototype) HIV-1 strains in vitro. Prototype strains are adapted to grow in continuous (neoplastic) cell lines and are more susceptible to neutralization than are primary isolates cultured in human peripheral blood mononuclear cells. In this study, 50 sera from nine phase I vaccine trials and 16 from HIV-1-infected persons were evaluated for neutralizing antibody activity against 3 laboratory-adapted and 5 primary HIV-1 isolates. Of 50 sera, 49 neutralized at least 1 of the prototype strains: however, none displayed neutralizing activity against primary isolates of HIV-1. Serum from most HIV- 1-infected persons neutralized both laboratory-adapted and primary HIV-1 isolates. These data demonstrate a qualitative, or large quantitative, difference in the neutralizing antibody response induced by envelope subunit vaccination and natural HIV-1 infection.",
author = "Mascola, {John R.} and Snyder, {Stuart W.} and Weislow, {Owen S.} and Belay, {Seifu M.} and Belshe, {Robert B.} and Schwartz, {David H.} and Clements, {Mary Lou} and Raphael Dolin and Graham, {Barney S.} and Gorse, {Geoffrey J.} and Keefer, {Michael C.} and McElrath, {M. Juliana} and Walker, {Mary Clare} and Wagner, {Kenneth F.} and McNeil, {John G.} and McCutchan, {Francine E.} and Burke, {Donald S.}",
note = "Funding Information: Division ofRetrovirology, Walter Reed Army Institute ofResearch, Henry M. Jackson Foundation for the Advancement ofMilitary Medicine. and SRA Technologies. Inc., Rockville, Department of Infectious Diseases, Naval Medical Research Institute. and National Institute ofAllergy and Infectious Diseases, National Institutes of Health, Bethesda, and Johns Hopkins School ofHygiene and Public Health and School ofMedicine, Baltimore, Maryland; Department of Medicine, Saint Louis University School ofMedicine, St. Louis, Missouri; Department ofMedicine, University ofRochester School of Medicine and Dentistry, New York, New York; Department of Medicine. Vanderbilt University School ofMedicine, Nashville, Tennessee; Department ofMedicine, University ofWashington School ofMedicine. Seattle. Washington Funding Information: Financial support: US Army Medical Research and Materiel Command; National Institutes of Health (AI-05061, -05063, -05064, -05065, -05062, and -52500).",
year = "1996",
doi = "10.1093/infdis/173.2.340",
language = "English (US)",
volume = "173",
pages = "340--348",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "2",
}