Immunization with AgTRIO, a Protein in Anopheles Saliva, Contributes to Protection against Plasmodium Infection in Mice

Srdjan M. Dragovic, Tolulope A. Agunbiade, Marianna Freudzon, Jing Yang, Andrew K. Hastings, Tyler R. Schleicher, Xia Zhou, Sam Craft, Yu Min Chuang, Floricel Gonzalez, Youquan Li, Gabriela Hrebikova, Abhai Tripathi, Godfree Mlambo, Lionel Almeras, Alexander Ploss, George Dimopoulos, Erol Fikrig

Research output: Contribution to journalArticle

Abstract

Plasmodium infection begins with the bite of an anopheline mosquito, when sporozoites along with saliva are injected into a vertebrate host. The role of the host responses to mosquito saliva components in malaria remains unclear. We observed that antisera against Anopheles gambiae salivary glands partially protected mice from mosquito-borne Plasmodium infection. Specifically, antibodies to A. gambiae TRIO (AgTRIO), a mosquito salivary gland antigen, contributed to the protection. Mice administered AgTRIO antiserum showed lower Plasmodium liver burden and decreased parasitemia when exposed to infected mosquitoes. Active immunization with AgTRIO was also partially protective against Plasmodium berghei infection. A combination of AgTRIO antiserum and antibodies against Plasmodium circumsporozoite protein, a vaccine candidate, further decreased P. berghei infection. In humanized mice, AgTRIO antiserum afforded some protection against mosquito-transmitted Plasmodium falciparum. AgTRIO antiserum reduced the movement of sporozoites in the murine dermis. AgTRIO may serve as an arthropod-based target against Plasmodium to combat malaria. Plasmodium infection begins with the bite of an anopheline mosquito, when sporozoites along with saliva are injected into a vertebrate host. Dragovic et al. demonstrate that antiserum against mosquito salivary glands decreases Plasmodium infection levels in mice, and antibodies against AgTRIO, a mosquito salivary protein, contribute to this effect.

Original languageEnglish (US)
Pages (from-to)523-535.e5
JournalCell Host and Microbe
Volume23
Issue number4
DOIs
StatePublished - Apr 11 2018

Fingerprint

Anopheles gambiae
Anopheles
Culicidae
Saliva
Malaria
Immunization
Immune Sera
Sporozoites
Proteins
Salivary Glands
Plasmodium berghei
Plasmodium
Bites and Stings
Vertebrates
Antibodies
Salivary Proteins and Peptides
Parasitemia
Arthropods
Plasmodium falciparum
Dermis

Keywords

  • AgTRIO
  • Anopheles mosquito
  • immunity
  • malaria
  • Plasmodium
  • saliva

ASJC Scopus subject areas

  • Parasitology
  • Microbiology
  • Virology

Cite this

Dragovic, S. M., Agunbiade, T. A., Freudzon, M., Yang, J., Hastings, A. K., Schleicher, T. R., ... Fikrig, E. (2018). Immunization with AgTRIO, a Protein in Anopheles Saliva, Contributes to Protection against Plasmodium Infection in Mice. Cell Host and Microbe, 23(4), 523-535.e5. https://doi.org/10.1016/j.chom.2018.03.008

Immunization with AgTRIO, a Protein in Anopheles Saliva, Contributes to Protection against Plasmodium Infection in Mice. / Dragovic, Srdjan M.; Agunbiade, Tolulope A.; Freudzon, Marianna; Yang, Jing; Hastings, Andrew K.; Schleicher, Tyler R.; Zhou, Xia; Craft, Sam; Chuang, Yu Min; Gonzalez, Floricel; Li, Youquan; Hrebikova, Gabriela; Tripathi, Abhai; Mlambo, Godfree; Almeras, Lionel; Ploss, Alexander; Dimopoulos, George; Fikrig, Erol.

In: Cell Host and Microbe, Vol. 23, No. 4, 11.04.2018, p. 523-535.e5.

Research output: Contribution to journalArticle

Dragovic, SM, Agunbiade, TA, Freudzon, M, Yang, J, Hastings, AK, Schleicher, TR, Zhou, X, Craft, S, Chuang, YM, Gonzalez, F, Li, Y, Hrebikova, G, Tripathi, A, Mlambo, G, Almeras, L, Ploss, A, Dimopoulos, G & Fikrig, E 2018, 'Immunization with AgTRIO, a Protein in Anopheles Saliva, Contributes to Protection against Plasmodium Infection in Mice', Cell Host and Microbe, vol. 23, no. 4, pp. 523-535.e5. https://doi.org/10.1016/j.chom.2018.03.008
Dragovic, Srdjan M. ; Agunbiade, Tolulope A. ; Freudzon, Marianna ; Yang, Jing ; Hastings, Andrew K. ; Schleicher, Tyler R. ; Zhou, Xia ; Craft, Sam ; Chuang, Yu Min ; Gonzalez, Floricel ; Li, Youquan ; Hrebikova, Gabriela ; Tripathi, Abhai ; Mlambo, Godfree ; Almeras, Lionel ; Ploss, Alexander ; Dimopoulos, George ; Fikrig, Erol. / Immunization with AgTRIO, a Protein in Anopheles Saliva, Contributes to Protection against Plasmodium Infection in Mice. In: Cell Host and Microbe. 2018 ; Vol. 23, No. 4. pp. 523-535.e5.
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