Immunization of neonatal mice with LAMP/p55 HIV gag DNA elicits robust immune responses that last to adulthood

Paula Ordonhez Rigato, Milton Maciel, Adriana Letícia Goldoni, Orlando Piubelli, Cyro Alves de Brito, Ana Elisa Fusaro, Liciana Xavier Eurico de Alencar, Thomas August, Ernesto Torres Azevedo Marques, Alberto José da Silva Duarte, Maria Notomi Sato

Research output: Contribution to journalArticle

Abstract

Successful T cell priming in early postnatal life that can generate effective long-lasting responses until adulthood is critical in HIV vaccination strategies because it prevents early sexual initiation and breastfeeding transmission of HIV. A chimeric DNA vaccine encoding p55 HIV gag associated with lysosome-associated membrane protein 1 (LAMP-1; which drives the antigen to the MIIC compartment), has been used to enhance cellular and humoral antigen-specific responses in adult mice and macaques. Herein, we investigated LAMP-1/gag vaccine immunogenicity in the neonatal period in mice and its ability to generate long-lasting effects. Neonatal vaccination with chimeric LAMP/gag generated stronger Gag-specific immune responses, as measured by the breadth of the Gag peptide-specific IFN-γ, proliferative responsiveness, cytokine production and antibody production, all of which revealed activation of CD4+ T cells as well as the generation of a more robust CTL response compared to gag vaccine alone. To induce long-lived T and B cell memory responses, it was necessary to immunize neonates with the chimeric LAMP/gag DNA vaccine. The LAMP/gag DNA vaccine strategy could be particularly useful for generating an anti-HIV immune response in the early postnatal period capable of inducing long-term immunological memory.

Original languageEnglish (US)
Pages (from-to)37-47
Number of pages11
JournalVirology
Volume406
Issue number1
DOIs
StatePublished - Oct 2010

Keywords

  • DNA vaccine
  • HIV-1
  • Immunological memory
  • Mice
  • Neonatal

ASJC Scopus subject areas

  • Virology

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