Immunization of HIV-infected patients with RGP160: Modulation of anti-RGP 120 antibody spectrotype

Roberto Biselli, Lawrence D. Loomis, Valerio Del Bono, Donald S. Burke, Robert R. Redfield, Deborah L. Birx

Research output: Contribution to journalArticle

Abstract

HIV-1 infection results in progressive failure of the immune system with decline in the number and/or function of B-cell clones originally recruited in specific humoral responses. Spectrotypic analysis, done by isoelectric focusing and reverse blotting (IEF-RB), is one technique for evaluating the activity and the number of specific B-cell clones and is Actaptable to the direct measurement of antibodies to conformationally intact epitopes. The anti-HIV-1 (IIIB) rgp 120 spectrotype was measured in 30 early-stage HIV-infected volunteers undergoing vaccine therapy with recombinant gp 160 (rgp 160). Twenty-five of the patients displayed a clear oligoclonal banding pattern; seven (28%) showed the same pattern in all samples, while 18 (72%) showed changes. Ten of the latter had an increase in band intensity over the course of immunization, and eight had an increase in both band intensity and number of bands. In contrast, serum samples from eight patients receiving placebo (alum) showed no changes over a comparable period. These findings suggest that vaccine therapy with rgp 160 may be able to expand the anti-HIV-1 (LAI) gp 120 B-cell clone pool in some HIV-infected patients as well as increase antibody synthesis by established B-cell clones recruited during natural infection. These data provide further evidence that postinfection vaccination may provide an alternative strategy in the treatment of chronic viral diseases.

Original languageEnglish (US)
Pages (from-to)1016-1024
Number of pages9
JournalJournal of Acquired Immune Deficiency Syndromes
Volume7
Issue number10
StatePublished - 1994
Externally publishedYes

Fingerprint

Immunization
B-Lymphocytes
Clone Cells
HIV
HIV-1
Active Immunotherapy
Antibodies
Isoelectric Focusing
Virus Diseases
HIV Infections
Epitopes
Volunteers
Immune System
Vaccination
Chronic Disease
Placebos
VaxSyn HIV-1 (gp160) vaccine
Infection
Serum
Therapeutics

Keywords

  • Envelope glycoprotein
  • Human immunodeficiency virus
  • Isoelectric focusing
  • Vaccine therapy

ASJC Scopus subject areas

  • Infectious Diseases
  • Pharmacology (medical)
  • Immunology and Allergy
  • Virology

Cite this

Biselli, R., Loomis, L. D., Del Bono, V., Burke, D. S., Redfield, R. R., & Birx, D. L. (1994). Immunization of HIV-infected patients with RGP160: Modulation of anti-RGP 120 antibody spectrotype. Journal of Acquired Immune Deficiency Syndromes, 7(10), 1016-1024.

Immunization of HIV-infected patients with RGP160 : Modulation of anti-RGP 120 antibody spectrotype. / Biselli, Roberto; Loomis, Lawrence D.; Del Bono, Valerio; Burke, Donald S.; Redfield, Robert R.; Birx, Deborah L.

In: Journal of Acquired Immune Deficiency Syndromes, Vol. 7, No. 10, 1994, p. 1016-1024.

Research output: Contribution to journalArticle

Biselli, R, Loomis, LD, Del Bono, V, Burke, DS, Redfield, RR & Birx, DL 1994, 'Immunization of HIV-infected patients with RGP160: Modulation of anti-RGP 120 antibody spectrotype', Journal of Acquired Immune Deficiency Syndromes, vol. 7, no. 10, pp. 1016-1024.
Biselli, Roberto ; Loomis, Lawrence D. ; Del Bono, Valerio ; Burke, Donald S. ; Redfield, Robert R. ; Birx, Deborah L. / Immunization of HIV-infected patients with RGP160 : Modulation of anti-RGP 120 antibody spectrotype. In: Journal of Acquired Immune Deficiency Syndromes. 1994 ; Vol. 7, No. 10. pp. 1016-1024.
@article{21961073370b4578abd53b95542af1d7,
title = "Immunization of HIV-infected patients with RGP160: Modulation of anti-RGP 120 antibody spectrotype",
abstract = "HIV-1 infection results in progressive failure of the immune system with decline in the number and/or function of B-cell clones originally recruited in specific humoral responses. Spectrotypic analysis, done by isoelectric focusing and reverse blotting (IEF-RB), is one technique for evaluating the activity and the number of specific B-cell clones and is Actaptable to the direct measurement of antibodies to conformationally intact epitopes. The anti-HIV-1 (IIIB) rgp 120 spectrotype was measured in 30 early-stage HIV-infected volunteers undergoing vaccine therapy with recombinant gp 160 (rgp 160). Twenty-five of the patients displayed a clear oligoclonal banding pattern; seven (28{\%}) showed the same pattern in all samples, while 18 (72{\%}) showed changes. Ten of the latter had an increase in band intensity over the course of immunization, and eight had an increase in both band intensity and number of bands. In contrast, serum samples from eight patients receiving placebo (alum) showed no changes over a comparable period. These findings suggest that vaccine therapy with rgp 160 may be able to expand the anti-HIV-1 (LAI) gp 120 B-cell clone pool in some HIV-infected patients as well as increase antibody synthesis by established B-cell clones recruited during natural infection. These data provide further evidence that postinfection vaccination may provide an alternative strategy in the treatment of chronic viral diseases.",
keywords = "Envelope glycoprotein, Human immunodeficiency virus, Isoelectric focusing, Vaccine therapy",
author = "Roberto Biselli and Loomis, {Lawrence D.} and {Del Bono}, Valerio and Burke, {Donald S.} and Redfield, {Robert R.} and Birx, {Deborah L.}",
year = "1994",
language = "English (US)",
volume = "7",
pages = "1016--1024",
journal = "Journal of Acquired Immune Deficiency Syndromes",
issn = "1525-4135",
publisher = "Lippincott Williams and Wilkins",
number = "10",

}

TY - JOUR

T1 - Immunization of HIV-infected patients with RGP160

T2 - Modulation of anti-RGP 120 antibody spectrotype

AU - Biselli, Roberto

AU - Loomis, Lawrence D.

AU - Del Bono, Valerio

AU - Burke, Donald S.

AU - Redfield, Robert R.

AU - Birx, Deborah L.

PY - 1994

Y1 - 1994

N2 - HIV-1 infection results in progressive failure of the immune system with decline in the number and/or function of B-cell clones originally recruited in specific humoral responses. Spectrotypic analysis, done by isoelectric focusing and reverse blotting (IEF-RB), is one technique for evaluating the activity and the number of specific B-cell clones and is Actaptable to the direct measurement of antibodies to conformationally intact epitopes. The anti-HIV-1 (IIIB) rgp 120 spectrotype was measured in 30 early-stage HIV-infected volunteers undergoing vaccine therapy with recombinant gp 160 (rgp 160). Twenty-five of the patients displayed a clear oligoclonal banding pattern; seven (28%) showed the same pattern in all samples, while 18 (72%) showed changes. Ten of the latter had an increase in band intensity over the course of immunization, and eight had an increase in both band intensity and number of bands. In contrast, serum samples from eight patients receiving placebo (alum) showed no changes over a comparable period. These findings suggest that vaccine therapy with rgp 160 may be able to expand the anti-HIV-1 (LAI) gp 120 B-cell clone pool in some HIV-infected patients as well as increase antibody synthesis by established B-cell clones recruited during natural infection. These data provide further evidence that postinfection vaccination may provide an alternative strategy in the treatment of chronic viral diseases.

AB - HIV-1 infection results in progressive failure of the immune system with decline in the number and/or function of B-cell clones originally recruited in specific humoral responses. Spectrotypic analysis, done by isoelectric focusing and reverse blotting (IEF-RB), is one technique for evaluating the activity and the number of specific B-cell clones and is Actaptable to the direct measurement of antibodies to conformationally intact epitopes. The anti-HIV-1 (IIIB) rgp 120 spectrotype was measured in 30 early-stage HIV-infected volunteers undergoing vaccine therapy with recombinant gp 160 (rgp 160). Twenty-five of the patients displayed a clear oligoclonal banding pattern; seven (28%) showed the same pattern in all samples, while 18 (72%) showed changes. Ten of the latter had an increase in band intensity over the course of immunization, and eight had an increase in both band intensity and number of bands. In contrast, serum samples from eight patients receiving placebo (alum) showed no changes over a comparable period. These findings suggest that vaccine therapy with rgp 160 may be able to expand the anti-HIV-1 (LAI) gp 120 B-cell clone pool in some HIV-infected patients as well as increase antibody synthesis by established B-cell clones recruited during natural infection. These data provide further evidence that postinfection vaccination may provide an alternative strategy in the treatment of chronic viral diseases.

KW - Envelope glycoprotein

KW - Human immunodeficiency virus

KW - Isoelectric focusing

KW - Vaccine therapy

UR - http://www.scopus.com/inward/record.url?scp=0028167726&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028167726&partnerID=8YFLogxK

M3 - Article

C2 - 8083819

AN - SCOPUS:0028167726

VL - 7

SP - 1016

EP - 1024

JO - Journal of Acquired Immune Deficiency Syndromes

JF - Journal of Acquired Immune Deficiency Syndromes

SN - 1525-4135

IS - 10

ER -