TY - JOUR
T1 - Immunity to experimental cholera. II. Secretory and humoral antitoxin response to local and systemic toxoid administration
AU - Pierce, N. F.
AU - Reynolds, H. Y.
N1 - Funding Information:
Received for publication July 1, 1974, and in revised form November 11, 1974. This work was supported in part by research grant no. AI-07625 and research contract no. 70-2051 from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. Dr. Pierce is the recipient of a Research Career Development Award from the National Institute of Allergy and Infectious Diseases, National Institutes of Health. We thank Mr. William Cray, Mr. Kenneth Rent, and Ms. Evelyn Kaniecki for their excellent technical assistance. We acknowledge the research facilities provided by the Gerontology Research Center of the National Institute of Child Health and Human Development under its Guest Scientist program. Please address requests for reprints to Dr. Nathaniel F. Pierce, Department of Medicine, Baltimore City Hospitals, 4940 Eastern Avenue, Baltimore, Maryland 21224.
PY - 1975
Y1 - 1975
N2 - The effect of parenteral or intrajejunal administration of formalinized cholera toxoid on antitoxin titers in serum and jejunal washings was studied in dogs with chronic Thiry Vella jejunal loops. In previously immunized dogs toxoid given by either route caused elevated titers of antitoxin in jejunal washings. After parenteral boosting, much of the antitoxin in jejunal washings was probably derived from serum, whereas after intrajejunal boosting, it appeared to be locally derived. In either instance, IgA antitoxin contributed to the intestinal immune response and had the characteristics of secretory IgA. In previously unimmunized dogs jejunal wash antitoxin was not demonstrable after a primary series of intrajejunal toxoid administrations but was demonstrable when the series was repeated four weeks later. After a single intrajejunal toxoid booster, antitoxin was demonstrable in washings within one to three days and peaked at seven days. Thus, the jejunal secretory immunologic system apparently has immunologic memory and is capable of mounting a rapid and brief secondary type response to locally administered antigen.
AB - The effect of parenteral or intrajejunal administration of formalinized cholera toxoid on antitoxin titers in serum and jejunal washings was studied in dogs with chronic Thiry Vella jejunal loops. In previously immunized dogs toxoid given by either route caused elevated titers of antitoxin in jejunal washings. After parenteral boosting, much of the antitoxin in jejunal washings was probably derived from serum, whereas after intrajejunal boosting, it appeared to be locally derived. In either instance, IgA antitoxin contributed to the intestinal immune response and had the characteristics of secretory IgA. In previously unimmunized dogs jejunal wash antitoxin was not demonstrable after a primary series of intrajejunal toxoid administrations but was demonstrable when the series was repeated four weeks later. After a single intrajejunal toxoid booster, antitoxin was demonstrable in washings within one to three days and peaked at seven days. Thus, the jejunal secretory immunologic system apparently has immunologic memory and is capable of mounting a rapid and brief secondary type response to locally administered antigen.
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U2 - 10.1093/infdis/131.4.383
DO - 10.1093/infdis/131.4.383
M3 - Article
C2 - 1117195
AN - SCOPUS:0016694152
SN - 0022-1899
VL - 131
SP - 383
EP - 389
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -