Immune response-related genes associated to blocking midgut dengue virus infection in Aedes aegypti strains that differ in susceptibility

Paola A. Caicedo, Idalba Mildred Serrato, Shuzhen Sim, George Dimopoulos, Heather Coatsworth, Carl Lowenberger, Clara B. Ocampo

Research output: Contribution to journalArticle

Abstract

Aedes (Stegomyia) aegypti, the principal global vector of dengue viruses, has differences in its susceptibility to dengue virus infection. We compared the global expression of genes in the midguts of Colombian Ae. aegypti dengue-susceptible (Cali-S) and dengue-refractory (Cali-MIB) field derived strains after ingesting either a sugarmeal, a bloodmeal, or a bloodmeal containing dengue virus serotype 2 (DENV-2). Microarray-based transcriptome analysis among treatments indicated a total of 4725 transcripts with differential expression between the two strains. Eleven genes were selected from different functional groups based on their significant up or down expression levels as well as reports in the literature suggesting they are associated with dengue virus elimination. We measured mRNA abundance of these 11 genes at 0, 8, 24, and 36 h postinfection using quantitative real time PCR (qPCR) to confirm the microarray results and assess any temporal patterns. Four genes were selected (Gram-negative binding protein—GNBP [AAEL009176], Niemann Pick Type-C2—NPC2 [AAEL015136], Keratinocyte lectin [AAEL009842], and Cathepsin-b [AAEL007585]) for knockdown experiments using RNA interference (RNAi) methodology to determine the phenotype (DENV-2 susceptible or refractory). Silencing GNBP, Cathepsin-b and Keratinocyte lectin reduced the percentage of mosquitoes with disseminated virus in the Cali-S strain to 8%, 20%, and 12% respectively compared with 96% in the controls. Silencing of NPC2 increased the percentage of mosquitos with disseminated virus infections in Cali-MIB to 66% compared with 35% in the controls. This study provides insight into genes that may contribute to the Cali-S susceptible and Cali-MIB refractory phenotypes in Ae. aegypti.

Original languageEnglish (US)
Pages (from-to)635-648
Number of pages14
JournalInsect Science
Volume26
Issue number4
DOIs
StatePublished - Aug 1 2019

Fingerprint

Dengue virus
Dengue Virus
Aedes
Virus Diseases
Aedes aegypti
immune response
Viruses
midgut
virus
Genes
gene
cathepsins
Cathepsins
infection
dengue
Dengue
keratinocytes
Refractory materials
Culicidae
Keratinocytes

Keywords

  • Aedes aegypti
  • dengue virus
  • innate immune response
  • knockdown
  • microarray
  • vector competence

ASJC Scopus subject areas

  • Ecology, Evolution, Behavior and Systematics
  • Agronomy and Crop Science
  • Biochemistry, Genetics and Molecular Biology(all)
  • Insect Science

Cite this

Immune response-related genes associated to blocking midgut dengue virus infection in Aedes aegypti strains that differ in susceptibility. / Caicedo, Paola A.; Serrato, Idalba Mildred; Sim, Shuzhen; Dimopoulos, George; Coatsworth, Heather; Lowenberger, Carl; Ocampo, Clara B.

In: Insect Science, Vol. 26, No. 4, 01.08.2019, p. 635-648.

Research output: Contribution to journalArticle

Caicedo, Paola A. ; Serrato, Idalba Mildred ; Sim, Shuzhen ; Dimopoulos, George ; Coatsworth, Heather ; Lowenberger, Carl ; Ocampo, Clara B. / Immune response-related genes associated to blocking midgut dengue virus infection in Aedes aegypti strains that differ in susceptibility. In: Insect Science. 2019 ; Vol. 26, No. 4. pp. 635-648.
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