Immune response following H1N1pdm09 vaccination: Differences in antibody repertoire and avidity in young adults and elderly populations stratified by age and gender

Surender Khurana, Nitin Verma, Kawsar Rasmy Talaat, Ruth A Karron, Hana Golding

Research output: Contribution to journalArticle

Abstract

Background.The H1N1 2009 influenza (H1N1pdm09) pandemic had unexpected features, including lower morbidity and mortality in elderly populations.Methods.We performed in-depth elucidation of antibody responses generated post-H1N1pdm09 vaccination in elderly (aged 66-83 years) and younger (aged 18-45 or 46-65 years) adults using H1N1pdm09 whole-genome-fragment phage display library and measured antibody isotype and affinity to antigenic domains within hemagglutinin (HA).Results.H1N1pdm09 vaccination induced 10-fold higher antibody levels in elderly compared with younger adults. These antibodies primarily targeted the HA1 globular domain, including neutralizing epitopes in the receptor-binding domain. Antibody epitope repertoire, isotype, and affinity maturation after H1N1pdm09 vaccination evolved independently for HA2, HA1, and HA1 N-terminus antigenic regions. Postvaccination serum samples from elderly subjects demonstrated substantially higher avidity than from younger subjects (>60% vs

Original languageEnglish (US)
Pages (from-to)610-620
Number of pages11
JournalJournal of Infectious Diseases
Volume205
Issue number4
DOIs
StatePublished - Feb 15 2012

Fingerprint

Antibody Affinity
Young Adult
Vaccination
Population
Antibodies
Epitopes
Hemagglutinins
Pandemics
Bacteriophages
Human Influenza
Libraries
Antibody Formation
Genome
Morbidity
Mortality
Serum

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

Cite this

@article{21f4a205ab8743b5ab65db37da69bb49,
title = "Immune response following H1N1pdm09 vaccination: Differences in antibody repertoire and avidity in young adults and elderly populations stratified by age and gender",
abstract = "Background.The H1N1 2009 influenza (H1N1pdm09) pandemic had unexpected features, including lower morbidity and mortality in elderly populations.Methods.We performed in-depth elucidation of antibody responses generated post-H1N1pdm09 vaccination in elderly (aged 66-83 years) and younger (aged 18-45 or 46-65 years) adults using H1N1pdm09 whole-genome-fragment phage display library and measured antibody isotype and affinity to antigenic domains within hemagglutinin (HA).Results.H1N1pdm09 vaccination induced 10-fold higher antibody levels in elderly compared with younger adults. These antibodies primarily targeted the HA1 globular domain, including neutralizing epitopes in the receptor-binding domain. Antibody epitope repertoire, isotype, and affinity maturation after H1N1pdm09 vaccination evolved independently for HA2, HA1, and HA1 N-terminus antigenic regions. Postvaccination serum samples from elderly subjects demonstrated substantially higher avidity than from younger subjects (>60{\%} vs",
author = "Surender Khurana and Nitin Verma and Talaat, {Kawsar Rasmy} and Karron, {Ruth A} and Hana Golding",
year = "2012",
month = "2",
day = "15",
doi = "10.1093/infdis/jir791",
language = "English (US)",
volume = "205",
pages = "610--620",
journal = "Journal of Infectious Diseases",
issn = "0022-1899",
publisher = "Oxford University Press",
number = "4",

}

TY - JOUR

T1 - Immune response following H1N1pdm09 vaccination

T2 - Differences in antibody repertoire and avidity in young adults and elderly populations stratified by age and gender

AU - Khurana, Surender

AU - Verma, Nitin

AU - Talaat, Kawsar Rasmy

AU - Karron, Ruth A

AU - Golding, Hana

PY - 2012/2/15

Y1 - 2012/2/15

N2 - Background.The H1N1 2009 influenza (H1N1pdm09) pandemic had unexpected features, including lower morbidity and mortality in elderly populations.Methods.We performed in-depth elucidation of antibody responses generated post-H1N1pdm09 vaccination in elderly (aged 66-83 years) and younger (aged 18-45 or 46-65 years) adults using H1N1pdm09 whole-genome-fragment phage display library and measured antibody isotype and affinity to antigenic domains within hemagglutinin (HA).Results.H1N1pdm09 vaccination induced 10-fold higher antibody levels in elderly compared with younger adults. These antibodies primarily targeted the HA1 globular domain, including neutralizing epitopes in the receptor-binding domain. Antibody epitope repertoire, isotype, and affinity maturation after H1N1pdm09 vaccination evolved independently for HA2, HA1, and HA1 N-terminus antigenic regions. Postvaccination serum samples from elderly subjects demonstrated substantially higher avidity than from younger subjects (>60% vs

AB - Background.The H1N1 2009 influenza (H1N1pdm09) pandemic had unexpected features, including lower morbidity and mortality in elderly populations.Methods.We performed in-depth elucidation of antibody responses generated post-H1N1pdm09 vaccination in elderly (aged 66-83 years) and younger (aged 18-45 or 46-65 years) adults using H1N1pdm09 whole-genome-fragment phage display library and measured antibody isotype and affinity to antigenic domains within hemagglutinin (HA).Results.H1N1pdm09 vaccination induced 10-fold higher antibody levels in elderly compared with younger adults. These antibodies primarily targeted the HA1 globular domain, including neutralizing epitopes in the receptor-binding domain. Antibody epitope repertoire, isotype, and affinity maturation after H1N1pdm09 vaccination evolved independently for HA2, HA1, and HA1 N-terminus antigenic regions. Postvaccination serum samples from elderly subjects demonstrated substantially higher avidity than from younger subjects (>60% vs

UR - http://www.scopus.com/inward/record.url?scp=84856297845&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84856297845&partnerID=8YFLogxK

U2 - 10.1093/infdis/jir791

DO - 10.1093/infdis/jir791

M3 - Article

C2 - 22207649

AN - SCOPUS:84856297845

VL - 205

SP - 610

EP - 620

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 4

ER -