Immune response following H1N1pdm09 vaccination: Differences in antibody repertoire and avidity in young adults and elderly populations stratified by age and gender

Surender Khurana, Nitin Verma, Kawsar Rasmy Talaat, Ruth A Karron, Hana Golding

Research output: Contribution to journalArticle

Abstract

Background.The H1N1 2009 influenza (H1N1pdm09) pandemic had unexpected features, including lower morbidity and mortality in elderly populations.Methods.We performed in-depth elucidation of antibody responses generated post-H1N1pdm09 vaccination in elderly (aged 66-83 years) and younger (aged 18-45 or 46-65 years) adults using H1N1pdm09 whole-genome-fragment phage display library and measured antibody isotype and affinity to antigenic domains within hemagglutinin (HA).Results.H1N1pdm09 vaccination induced 10-fold higher antibody levels in elderly compared with younger adults. These antibodies primarily targeted the HA1 globular domain, including neutralizing epitopes in the receptor-binding domain. Antibody epitope repertoire, isotype, and affinity maturation after H1N1pdm09 vaccination evolved independently for HA2, HA1, and HA1 N-terminus antigenic regions. Postvaccination serum samples from elderly subjects demonstrated substantially higher avidity than from younger subjects (>60% vs

Original languageEnglish (US)
Pages (from-to)610-620
Number of pages11
JournalJournal of Infectious Diseases
Volume205
Issue number4
DOIs
Publication statusPublished - Feb 15 2012

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ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy

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