Immune regulation of the L5178Y murine tumor-dormant state

Induction of cell- and soluble factor-mediated inhibition of tumor cell growth by tumor necrosis factor and γ-interferon

Y. Suzuki, L. Chen, C. M. Liu, T. Morita, E. F. Wheelock

Research output: Contribution to journalArticle

Abstract

Small concentrations of recombinant murine tumor necrosis factor (rMuTNF) synergized with recombinant murine γ-interferon (rMuIFN-γ) to inhibit tumor cell growth in peritoneal cell (PC) cultures from tumor-dormant mice. A soluble inhibitor of tumor cell growth was produced in the rMuTNF-treated PC cultures, but there was no synergistic enhancement of production of this inhibitor by the addition of rMuIFN-γ. Treatment of both plastic-adherent PC and nonadherent PC cultures which contained L5178Y cells with rMuTNF + rMuIFN-γ resulted in inhibition of tumor cell growth. However, in the absence of L5178Y cells, rMuTNF + rMuIFN-γ induced antitumor cytotoxic activity in the plastic-adherent but not in the plastic nonadherent PC. These results indicate that the antitumor activity of rMuTNF + rMuIFN-γ in PC from tumor-dormant mice involves both cell- and soluble factor-mediated mechanisms.

Original languageEnglish (US)
Pages (from-to)2028-2033
Number of pages6
JournalCancer Research
Volume49
Issue number8
StatePublished - 1989
Externally publishedYes

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Interferons
Tumor Necrosis Factor-alpha
Growth
Neoplasms
Plastics
Cell Culture Techniques
Growth Inhibitors

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Immune regulation of the L5178Y murine tumor-dormant state : Induction of cell- and soluble factor-mediated inhibition of tumor cell growth by tumor necrosis factor and γ-interferon. / Suzuki, Y.; Chen, L.; Liu, C. M.; Morita, T.; Wheelock, E. F.

In: Cancer Research, Vol. 49, No. 8, 1989, p. 2028-2033.

Research output: Contribution to journalArticle

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abstract = "Small concentrations of recombinant murine tumor necrosis factor (rMuTNF) synergized with recombinant murine γ-interferon (rMuIFN-γ) to inhibit tumor cell growth in peritoneal cell (PC) cultures from tumor-dormant mice. A soluble inhibitor of tumor cell growth was produced in the rMuTNF-treated PC cultures, but there was no synergistic enhancement of production of this inhibitor by the addition of rMuIFN-γ. Treatment of both plastic-adherent PC and nonadherent PC cultures which contained L5178Y cells with rMuTNF + rMuIFN-γ resulted in inhibition of tumor cell growth. However, in the absence of L5178Y cells, rMuTNF + rMuIFN-γ induced antitumor cytotoxic activity in the plastic-adherent but not in the plastic nonadherent PC. These results indicate that the antitumor activity of rMuTNF + rMuIFN-γ in PC from tumor-dormant mice involves both cell- and soluble factor-mediated mechanisms.",
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