Immune modulatory effects of IL-22 on allergen-induced pulmonary inflammation

Ping Fang, Li Zhou, Yuqi Zhou, Jay K. Kolls, Tao Zheng, Zhou Zhu

Research output: Contribution to journalArticle

Abstract

IL-22 is a Th17/Th22 cytokine that is increased in asthma. However, recent animal studies showed controversial findings in the effects of IL-22 in allergic asthma. To determine the role of IL-22 in ovalbumin-induced allergic inflammation we generated inducible lung-specific IL-22 transgenic mice. Transgenic IL-22 expression and signaling activity in the lung were determined. Ovalbumin (OVA)-induced pulmonary inflammation, immune responses, and airway hyperresponsiveness (AHR) were examined and compared between IL-22 transgenic mice and wild type controls. Following doxycycline (Dox) induction, IL-22 protein was readily detected in the large (CC10 promoter) and small (SPC promoter) airway epithelial cells. IL-22 signaling was evidenced by phosphorylated STAT3. After OVA sensitization and challenge, compared to wild type littermates, IL-22 transgenic mice showed decreased eosinophils in the bronchoalveolar lavage (BAL), and in lung tissue, decreased mucus metaplasia in the airways, and reduced AHR. Among the cytokines and chemokines examined, IL-13 levels were reduced in the BAL fluid as well as in lymphocytes from local draining lymph nodes of IL-22 transgenic mice. No effect was seen on the levels of serum total or OVA-specific IgE or IgG. These findings indicate that IL-22 has immune modulatory effects on pulmonary inflammatory responses in allergen-induced asthma.

Original languageEnglish (US)
Article numbere107454
JournalPLoS One
Volume9
Issue number9
DOIs
StatePublished - Sep 25 2014

Fingerprint

allergens
Allergens
ovalbumin
Pneumonia
inflammation
lungs
genetically modified organisms
asthma
mice
Ovalbumin
Transgenic Mice
cytokines
promoter regions
metaplasia
doxycycline
Lung
Asthma
chemokines
eosinophils
mucus

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Fang, P., Zhou, L., Zhou, Y., Kolls, J. K., Zheng, T., & Zhu, Z. (2014). Immune modulatory effects of IL-22 on allergen-induced pulmonary inflammation. PLoS One, 9(9), [e107454]. https://doi.org/10.1371/journal.pone.0107454

Immune modulatory effects of IL-22 on allergen-induced pulmonary inflammation. / Fang, Ping; Zhou, Li; Zhou, Yuqi; Kolls, Jay K.; Zheng, Tao; Zhu, Zhou.

In: PLoS One, Vol. 9, No. 9, e107454, 25.09.2014.

Research output: Contribution to journalArticle

Fang, P, Zhou, L, Zhou, Y, Kolls, JK, Zheng, T & Zhu, Z 2014, 'Immune modulatory effects of IL-22 on allergen-induced pulmonary inflammation', PLoS One, vol. 9, no. 9, e107454. https://doi.org/10.1371/journal.pone.0107454
Fang, Ping ; Zhou, Li ; Zhou, Yuqi ; Kolls, Jay K. ; Zheng, Tao ; Zhu, Zhou. / Immune modulatory effects of IL-22 on allergen-induced pulmonary inflammation. In: PLoS One. 2014 ; Vol. 9, No. 9.
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