Immune CD4+ T cells specific for Theileria parva-infected lymphocytes recognize a 24-kilodalton protein

D. J. Grab, C. L. Baldwin, W. C. Brown, E. A. Innes, J. D. Lonsdale-Eccles, Y. Verjee

Research output: Contribution to journalArticle

Abstract

Theileria parva is a protozoan parasite that infects and transforms bovine lymphocytes. Here we report the partial purification of a T. parva-specific protein from infected lymphocytes that is recognized by CD4+ parasite- specific T-cell clones derived from immune cattle. T. parva-infected lymphocytes were homogenized in Dulbecco's phosphate-buffered saline in the presence of protease inhibitors. The antigen was purified from a postmicrosomal supernatant by using a combination of DEAE-cellulose chromatography and hydroxylapatite column chromatography. After labelling with 125I, the antigen preparation was subjected to sodium dodecyl sulfate-polyacrylamide gel electrophoresis and found to contain 8 to 10 proteins. This preparation was subjected to chromatography in phosphate- buffered saline on HPLC TSK-250/125 columns coupled in tandem. A radiolabelled protein of M(r) 24,000 correlated with antigenic activity.

Original languageEnglish (US)
Pages (from-to)3892-3896
Number of pages5
JournalInfection and Immunity
Volume60
Issue number9
StatePublished - 1992
Externally publishedYes

ASJC Scopus subject areas

  • Immunology

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    Grab, D. J., Baldwin, C. L., Brown, W. C., Innes, E. A., Lonsdale-Eccles, J. D., & Verjee, Y. (1992). Immune CD4+ T cells specific for Theileria parva-infected lymphocytes recognize a 24-kilodalton protein. Infection and Immunity, 60(9), 3892-3896.