Immune activation of platelets in response to serial phlebotomy in pigtailed macaques (Macaca nemestrina)

Meghan S. Vermillion, Claire E. Lyons, Kevin M. Najarro, Robert J. Adams, Kelly A. Metcalf Pate

Research output: Research - peer-reviewArticle

Abstract

Serial phlebotomy is a common sampling practice for repeated-measures studies in biomedical research. In NHP, the effect of serial blood collection on RBC parameters has been characterized, but the effects on platelet parameters and other aspects of the hemogram have not been well studied. We sought to characterize the circulating platelet phenotype throughout the course of 7 serial phlebotomies spanning 30 d in pigtailed macaques (Macaca nemestrina). Phlebotomy was performed on 23 animals at days 0, 2, 4, 7, 10, 21, and 30 to quantify the circulating platelet count and markers of both hemostatic and immune platelet activation. Platelet immune activation was characterized by increases in surface MHC class I and II expression and increases in circulating platelet-leukocyte aggregates. These changes occurred in the absence of increases in the prohemostatic markers P-selectin and CD40L and without evidence of adverse clinical effects. Mild increases in platelet count, mean platelet volume, and immune activation occurred early in the study. After day 21, mean platelet volume and other hematologic parameters returned to baseline while changes in platelet count and immune activation were greater than during the first 10 d of the study. These data demonstrate that serial phlebotomy in NHP has delayed effects on platelet parameters, which may be a source of clinically silent, immunologic and physiologic variability within repeated measures studies. The impact of these effects on research aims should be considered when designing protocols requiring serial phlebotomy in NHP.

LanguageEnglish (US)
Pages360-367
Number of pages8
JournalComparative Medicine
Volume67
Issue number4
StatePublished - Jul 1 2017

Fingerprint

Macaca nemestrina
Phlebotomy
Platelet Activation
Macaca
platelet activation
blood platelet count
Platelets
Chemical activation
Blood Platelets
Platelet Count
Mean Platelet Volume
biomedical research
cell aggregates
leukocytes
erythrocytes
phenotype
blood
animals
sampling
CD40 Ligand

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

Immune activation of platelets in response to serial phlebotomy in pigtailed macaques (Macaca nemestrina). / Vermillion, Meghan S.; Lyons, Claire E.; Najarro, Kevin M.; Adams, Robert J.; Metcalf Pate, Kelly A.

In: Comparative Medicine, Vol. 67, No. 4, 01.07.2017, p. 360-367.

Research output: Research - peer-reviewArticle

@article{c29581dd461c44a3811233a1afbc9428,
title = "Immune activation of platelets in response to serial phlebotomy in pigtailed macaques (Macaca nemestrina)",
abstract = "Serial phlebotomy is a common sampling practice for repeated-measures studies in biomedical research. In NHP, the effect of serial blood collection on RBC parameters has been characterized, but the effects on platelet parameters and other aspects of the hemogram have not been well studied. We sought to characterize the circulating platelet phenotype throughout the course of 7 serial phlebotomies spanning 30 d in pigtailed macaques (Macaca nemestrina). Phlebotomy was performed on 23 animals at days 0, 2, 4, 7, 10, 21, and 30 to quantify the circulating platelet count and markers of both hemostatic and immune platelet activation. Platelet immune activation was characterized by increases in surface MHC class I and II expression and increases in circulating platelet-leukocyte aggregates. These changes occurred in the absence of increases in the prohemostatic markers P-selectin and CD40L and without evidence of adverse clinical effects. Mild increases in platelet count, mean platelet volume, and immune activation occurred early in the study. After day 21, mean platelet volume and other hematologic parameters returned to baseline while changes in platelet count and immune activation were greater than during the first 10 d of the study. These data demonstrate that serial phlebotomy in NHP has delayed effects on platelet parameters, which may be a source of clinically silent, immunologic and physiologic variability within repeated measures studies. The impact of these effects on research aims should be considered when designing protocols requiring serial phlebotomy in NHP.",
author = "Vermillion, {Meghan S.} and Lyons, {Claire E.} and Najarro, {Kevin M.} and Adams, {Robert J.} and {Metcalf Pate}, {Kelly A.}",
year = "2017",
month = "7",
volume = "67",
pages = "360--367",
journal = "Comparative Medicine",
issn = "1532-0820",
publisher = "American Association for Laboratory Animal Science",
number = "4",

}

TY - JOUR

T1 - Immune activation of platelets in response to serial phlebotomy in pigtailed macaques (Macaca nemestrina)

AU - Vermillion,Meghan S.

AU - Lyons,Claire E.

AU - Najarro,Kevin M.

AU - Adams,Robert J.

AU - Metcalf Pate,Kelly A.

PY - 2017/7/1

Y1 - 2017/7/1

N2 - Serial phlebotomy is a common sampling practice for repeated-measures studies in biomedical research. In NHP, the effect of serial blood collection on RBC parameters has been characterized, but the effects on platelet parameters and other aspects of the hemogram have not been well studied. We sought to characterize the circulating platelet phenotype throughout the course of 7 serial phlebotomies spanning 30 d in pigtailed macaques (Macaca nemestrina). Phlebotomy was performed on 23 animals at days 0, 2, 4, 7, 10, 21, and 30 to quantify the circulating platelet count and markers of both hemostatic and immune platelet activation. Platelet immune activation was characterized by increases in surface MHC class I and II expression and increases in circulating platelet-leukocyte aggregates. These changes occurred in the absence of increases in the prohemostatic markers P-selectin and CD40L and without evidence of adverse clinical effects. Mild increases in platelet count, mean platelet volume, and immune activation occurred early in the study. After day 21, mean platelet volume and other hematologic parameters returned to baseline while changes in platelet count and immune activation were greater than during the first 10 d of the study. These data demonstrate that serial phlebotomy in NHP has delayed effects on platelet parameters, which may be a source of clinically silent, immunologic and physiologic variability within repeated measures studies. The impact of these effects on research aims should be considered when designing protocols requiring serial phlebotomy in NHP.

AB - Serial phlebotomy is a common sampling practice for repeated-measures studies in biomedical research. In NHP, the effect of serial blood collection on RBC parameters has been characterized, but the effects on platelet parameters and other aspects of the hemogram have not been well studied. We sought to characterize the circulating platelet phenotype throughout the course of 7 serial phlebotomies spanning 30 d in pigtailed macaques (Macaca nemestrina). Phlebotomy was performed on 23 animals at days 0, 2, 4, 7, 10, 21, and 30 to quantify the circulating platelet count and markers of both hemostatic and immune platelet activation. Platelet immune activation was characterized by increases in surface MHC class I and II expression and increases in circulating platelet-leukocyte aggregates. These changes occurred in the absence of increases in the prohemostatic markers P-selectin and CD40L and without evidence of adverse clinical effects. Mild increases in platelet count, mean platelet volume, and immune activation occurred early in the study. After day 21, mean platelet volume and other hematologic parameters returned to baseline while changes in platelet count and immune activation were greater than during the first 10 d of the study. These data demonstrate that serial phlebotomy in NHP has delayed effects on platelet parameters, which may be a source of clinically silent, immunologic and physiologic variability within repeated measures studies. The impact of these effects on research aims should be considered when designing protocols requiring serial phlebotomy in NHP.

UR - http://www.scopus.com/inward/record.url?scp=85028467564&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85028467564&partnerID=8YFLogxK

M3 - Article

VL - 67

SP - 360

EP - 367

JO - Comparative Medicine

T2 - Comparative Medicine

JF - Comparative Medicine

SN - 1532-0820

IS - 4

ER -