TY - JOUR
T1 - Immortalized neural stem cells differ from nonimmortalized cortical neurospheres and cerebellar granule cell progenitors
AU - Mi, Ruifa
AU - Luo, Yongquan
AU - Cai, Jingli
AU - Limke, Tobi L.
AU - Rao, Mahendra S.
AU - Höke, Ahmet
N1 - Funding Information:
We gratefully acknowledge the input of all members of our laboratories provided through discussions and constructive criticisms. Tobi L. Limke was supported by a Pharmacology Research Associate (PRAT) fellowship from NIGMS (NIH). Mahendra S. Rao was supported by the NIA (NIH), the Packard Center for ALS Research at Johns Hopkins and the CNS foundation. Ahmet Höke was supported by the NINDS, NIMH (NIH), and the Packard Center for ALS Research at Johns Hopkins.
PY - 2005/8
Y1 - 2005/8
N2 - Pluripotent neural stem cells (NSCs) have been used as replacement cells in a variety of neurological disease models. Among the many different NSCs that have been used to date, most robust results have been obtained with the immortalized neural stem cell line (C17.2) isolated from postnatal cerebellum. However, it is unclear if other NSCs isolated from different brain regions are similar in their potency as replacement therapies. To assess the properties of NSC-like C17.2 cells, we compared the properties of these cells with those reported for other NSC populations identified by a variety of different investigators using biological assays, microarray analysis, RT-PCR, and immunocytochemistry. We show that C17.2 cells differ significantly from other NSCs and cerebellar granule cell precursors, from which they were derived. In particular, they secrete additional growth factors and cytokines, express markers that distinguish them from other progenitor populations, and do not maintain karyotypic stability. Our results provide a caution on extrapolating results from C17.2 to other nonimmortalized stem cell populations and provide an explanation for some of the dramatic effects that are seen with C17.2 transplants but not with other cells. We suggest that, while C17.2 cells can illustrate many fundamental aspects of neural biology and are useful in their own right, their unique properties cannot be generalized.
AB - Pluripotent neural stem cells (NSCs) have been used as replacement cells in a variety of neurological disease models. Among the many different NSCs that have been used to date, most robust results have been obtained with the immortalized neural stem cell line (C17.2) isolated from postnatal cerebellum. However, it is unclear if other NSCs isolated from different brain regions are similar in their potency as replacement therapies. To assess the properties of NSC-like C17.2 cells, we compared the properties of these cells with those reported for other NSC populations identified by a variety of different investigators using biological assays, microarray analysis, RT-PCR, and immunocytochemistry. We show that C17.2 cells differ significantly from other NSCs and cerebellar granule cell precursors, from which they were derived. In particular, they secrete additional growth factors and cytokines, express markers that distinguish them from other progenitor populations, and do not maintain karyotypic stability. Our results provide a caution on extrapolating results from C17.2 to other nonimmortalized stem cell populations and provide an explanation for some of the dramatic effects that are seen with C17.2 transplants but not with other cells. We suggest that, while C17.2 cells can illustrate many fundamental aspects of neural biology and are useful in their own right, their unique properties cannot be generalized.
KW - C17.2 cells
KW - Neural stem cells
KW - Nonimmortalized stem cell population
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U2 - 10.1016/j.expneurol.2004.07.011
DO - 10.1016/j.expneurol.2004.07.011
M3 - Article
C2 - 16022860
AN - SCOPUS:22044436102
SN - 0014-4886
VL - 194
SP - 301
EP - 319
JO - Experimental Neurology
JF - Experimental Neurology
IS - 2
ER -