Abstract
Partially purified rabbit liver UDPglucuronosyltransferase is immobilized on agarose by the cyanogen bromide activation method. Both soluble and matrix-bound enzyme preparations display very similar Km and pH optimum. The storage stability of the immobilized enzyme at 4° is 5-10 times improved over the soluble preparations. The agarose-bound UDPglucuronosyltransferase is successfully used in the synthesis of p-nitrophenyl glucuronide in an overall yield of 50-70%. The matrix-bound enzyme is reusable over an extended period of time and offers an easy and convenient synthetic tool for various drug glucuronides.
Original language | English (US) |
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Pages (from-to) | 296-299 |
Number of pages | 4 |
Journal | Journal of medicinal chemistry |
Volume | 19 |
Issue number | 2 |
DOIs | |
State | Published - Feb 1 1976 |
ASJC Scopus subject areas
- Molecular Medicine
- Drug Discovery