Immobilized Glucuronosyltransferase for the Synthesis of Conjugates

Indu Parikh, Donald W. MacGlashan, Catherine Fenselau

Research output: Contribution to journalArticlepeer-review

Abstract

Partially purified rabbit liver UDPglucuronosyltransferase is immobilized on agarose by the cyanogen bromide activation method. Both soluble and matrix-bound enzyme preparations display very similar Km and pH optimum. The storage stability of the immobilized enzyme at 4° is 5-10 times improved over the soluble preparations. The agarose-bound UDPglucuronosyltransferase is successfully used in the synthesis of p-nitrophenyl glucuronide in an overall yield of 50-70%. The matrix-bound enzyme is reusable over an extended period of time and offers an easy and convenient synthetic tool for various drug glucuronides.

Original languageEnglish (US)
Pages (from-to)296-299
Number of pages4
JournalJournal of medicinal chemistry
Volume19
Issue number2
DOIs
StatePublished - Feb 1 1976

ASJC Scopus subject areas

  • Molecular Medicine
  • Drug Discovery

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